Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 61299..61889 Replicon plasmid pRHBSTW-00935_4
Accession NZ_CP056241
Organism Citrobacter freundii strain RHBSTW-00935

Toxin (Protein)


Gene name pemK Uniprot ID A0A0J1NBD3
Locus tag HV330_RS26885 Protein ID WP_003836692.1
Coordinates 61299..61631 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1NBD4
Locus tag HV330_RS26890 Protein ID WP_003836694.1
Coordinates 61632..61889 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV330_RS26860 57052..58539 + 1488 WP_003846749.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
HV330_RS26870 58855..59805 - 951 WP_003846751.1 HTH-type transcriptional regulator Cbl -
HV330_RS26875 59907..60824 - 918 WP_003030855.1 nitrogen assimilation transcriptional regulator NAC -
HV330_RS26885 61299..61631 - 333 WP_003836692.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HV330_RS26890 61632..61889 - 258 WP_003836694.1 hypothetical protein Antitoxin
HV330_RS26895 62613..63310 + 698 Protein_61 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - iutA 1..82097 82097
- flank IS/Tn - - 63017..63310 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11703.48 Da        Isoelectric Point: 8.5572

>T163091 WP_003836692.1 NZ_CP056241:c61631-61299 [Citrobacter freundii]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Download         Length: 333 bp

>T163091 NZ_CP072363:1683999-1685333 [Prevotella jejuni]
ATGAAAGATTTAGTTGTTACAATTTCGTTATATGGAAGGACAGTTGGCTATGCCTCTTGGGATAATAACAGAGGCTCTGC
ATCCTTTGAATTTGAACCAAAGTTTATTAATTCTGGTCTTAATGTTTCTCCCTTAATAATGCCGTTAGCAAAGTCCAAAG
GTAGAGTATATAATTTCCCAGAAAACATCAATAATTGTTTTGGTGGCCTACCTGGCATGCTCTCTGATTCATTACCAGAT
AAATATGGGTCAGAAAAGATCGATGCTTATTTTGCTTCAAAAGGATTACTAACCTCTGATGTAACACCTTTAGATAGACT
GTGTTATATCGGTAAGCGTGGAATGGGTGCATTGGAATTCGAGCCATCTAATAGTTATAAAGACCTAGAAGAGTCAAGTG
CTCTCGATATACAAGCTATTGTAGATGTAGCAAAAGAGATATTCCAAAGCAGAGAGATGTTTAAGGACTTACTTAATCAA
AAAGATAAACGAATTATCGACATTCTAAAGGTTGGGACATCGGCTGGAGGTGCAAAGCCAAAAGCTATTATAGCTTATAA
TGATGAAACGAATGAAGTTAGATCTGGTCAGGTGAAAGCACCAGAAGGATTTGGATACTGGCTTTTAAAATTTGATGGTG
GTGAGTTTCTTGAACATAACCAAATTTGCGAAAATCCTAAAGGAATAGGGCAGATAGAGTATGCTTATTATAAGATGGCT
AAAGCAGCTGGTATAGATATGAATGAGTGTCGCTTATTAGAGGAAGGAGATAGTCATCATTTTATGACACGACGCTTCGA
TCGAACAATGACTGGAGAGAAAATACACATGCAAACACTTGCTGCTATTGCACATTATAATCGGGATGAGAGACACTCCT
ACGAACAGCTGTTCGAAGTTATGAGAAAATTAGATCTTCCTTATACTGACTACAGAGAAATGTTTCGCCGAATGGTATTC
AATGTTGCAGCAAGAAATCATGATGATCATACTAAAAATCATTCTTTCTTAATGGATAGAAATGGTAAATGGTCTTTAGC
CCCAGCATATGACCTATGTTATTCTTACAATAAAAATGGGAAGTTTACAAAGTACCACCAGATGTTTCTCAATGGCAAAC
AGGACAATTTCACATATAATGATTTAAAAAATGTGGCAGACAACATTGGTATTAAATTCTCTGAGAATATTATAAACGAA
GTGATGGATGTTACTGCAGAATGGAAATCATTTGCTAAAGAGGCAGGTGTAAGAAGGGAATATTCAGAAGAAATTAATGA
TAATATTTTGTCACTAAAATCTTCTCCTGGTATTTCCAAGGGTATTAGAAGGTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9428.89 Da        Isoelectric Point: 4.3440

>AT163091 WP_003836694.1 NZ_CP056241:c61889-61632 [Citrobacter freundii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT163091 NZ_CP072363:1683697-1684002 [Prevotella jejuni]
ATGTGGAAAGAAAAGTCTAATCCCTCCATCTTGAAAGAGATAGGAAAGAGAATACAAAGAGAAAGGTTACGCCAAGACAT
CACTCAGGCCTGCTTGGCAGAATCATCAGGAGTAAGTTTGATAGTAGTCCAACGTATCGAGAAAGGTATGTCAATATCCC
TTCAGAACCTTCTGGGGATCCTCCGTGCTTTGAATATGTTAGAGGGGTTGGAACAACTTTTCCCAGAACCCCCTATAAGT
CCCATCTTACTTAAAAAACTTTCTGGTAAGACACGTAAGCGTGCTTCAAAAAATATATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NBD3

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1NBD4

References