Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 143999..144802 Replicon plasmid pRHBSTW-00935_2
Accession NZ_CP056239
Organism Citrobacter freundii strain RHBSTW-00935

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag HV330_RS25570 Protein ID WP_022652313.1
Coordinates 144272..144802 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag HV330_RS25565 Protein ID WP_022652312.1
Coordinates 143999..144268 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV330_RS25550 140304..140611 - 308 Protein_144 IS1 family transposase -
HV330_RS25555 141088..141828 + 741 WP_022652310.1 site-specific integrase -
HV330_RS25560 142154..143143 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
HV330_RS25565 143999..144268 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
HV330_RS25570 144272..144802 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
HV330_RS25575 144934..145827 + 894 WP_161348953.1 IS5 family transposase -
HV330_RS25580 145968..146444 - 477 Protein_150 IS3 family transposase -
HV330_RS25585 146525..148033 - 1509 WP_001189111.1 group II intron reverse transcriptase/maturase -
HV330_RS25590 148706..149135 - 430 Protein_152 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..206642 206642
- inside IScluster/Tn - - 138990..152196 13206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T163089 WP_022652313.1 NZ_CP056239:144272-144802 [Citrobacter freundii]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T163089 NZ_CP072326:c4564196-4562877 [Aeromonas caviae]
ATGGCCAATCTGGCTGTCTACATGAACGGCTATCGGGTAGGCACATTCACCAAAACTACCAGCGGTGCCCATCAGTTTCA
GTACCATGAGTCATGGCTAGCACAACCGGGCAGCCGTCCGATCTCACTCTCCATGCCATTGCGCCATCAGGCCTACCGGG
GCGATGAAGCCTATAACTTCTTCGACAACCTGCTGCCAGATAATACGGAGGTGAGGAACAGGGTTGTAGCACGCTATCAA
GCGGCATCAACTCAGCCCTTCGATCTGCTCAGTTGCATTGGCCAGGATAGCGTAGGGGCATTACAGCTGGTTACCGAAGG
ACACGATGTTCCAGATGTCAGGCGAATCGACTACAAGGCCCTTTCCGATGACGAGCTCAAGCAGATCCTGACCAGCTACA
AATCAGGAATCCCTTTGGGGATGGTCAGGGAACAAGAGGAGTTTCGGATCTCTATCGCCGGGGTGCAAGAAAAGACTGCG
CTGCTCTATCTCGATGGCCTTTGGTACCTGCCACTCAACGCAACGCCGACAACCCACATTATCAAACTCCCTATCGGCAA
GATTGAAAGCCACTCCTACTCGATAGACCTCTCCCAGAGCGTAGAAAACGAATATCTCTGCACTCTGATTGCCAAGGAGT
TTGGTCTACCCGTACCTCACTGCTTCATGATGCAGGTTGGCCAGGTCAAAGCACTGGCTGTTGAACGCTTCGACAGACGT
TACGCTGCCGATCGCAGTTGGATAATGCGCCTTCCCCAAGAAGATTTCTGTCAGGTATTGAATGTTCCGTCAGCACGGAA
ATACGAGAGCCATGGAGGACCAGGGATCACTGACATCATGGAAACCTTGCTGGGCTCTGCGACACCAGAACAGGATCGCT
ACCTGTTCATGAAATCTCAGGTCCTGTTCTGGCTGCTGGCAGCCACCGATGGCCATGCAAAGAACTTCTCGGTCTTTATT
GAACCGGAAGGGCGTTTTCGCCTGACCCCTTTTTACGACATCCTATCTGTCTATCCAGTGTTTGGCGGCCGAGGCCTCAA
CCGAAGGGACGCCAAGCTCGCTATGGGCCTAGCAAGCAGCAAGGGAAAGAAGTACTCCATTGAGCAGATATTCCCGCGTC
ATTTCTTCCAAACGGCAAAAACTGTGGGATTTGAGCGTTCTGCTATGGAAGAGATCCTGACAGAACTGGCCAACTCCGTT
GATGATGTTATTGAGCGCGTTACCCAGCAATTGCCAGCAGACTTCCCCGATACCATCAGCAGCACCATTCTAGAGGGCCT
AAAAGCCCGCTCAGCCAGGCTGATAAAGGGTTGGGATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT163089 WP_022652312.1 NZ_CP056239:143999-144268 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT163089 NZ_CP072326:c4564450-4564196 [Aeromonas caviae]
ATGAAAGTAACCAGCGCCAAGCAGCTGAGCGCCTGTATCAAGGATGCCAGGCTTTCTCAAAAGCTCTCTCAAGGCAAAGT
GGCCGAGAAAGTGGGGATTAGGCAAGACACAGTCTCCAGCTTCGAGCTCAATCCTGAGTCCACCAAGCTGGAAACCCTGT
TCAAGATCCTCGCAGCACTCGAATTGTCAGTGGATATCAAACCCCGCAATGAGTCATCCGAGAGTCCGGCATCGGGCTGG
AAGGAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References