Detailed information of TA system TA163088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 73849..74492
• Replicon: plasmid pRHBSTW-00935_2
• Accession: NZ_CP056239
• Organism: Citrobacter freundii strain RHBSTW-00935

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV330_RS25160
• Protein ID: WP_181502632.1
• Coordinates: 73849..74265 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6S5K7T9
• Locus tag: HV330_RS25165
• Protein ID: WP_059362947.1
• Coordinates: 74262..74492 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV330_RS25145	68860..69864	+	1005	WP_117273198.1	IS110-like element IS4321 family transposase	-
HV330_RS25150	70210..71050	+	841	Protein_64	EAL domain-containing protein	-
HV330_RS25155	72203..73147	-	945	WP_181502633.1	sensor domain-containing diguanylate cyclase	-
HV330_RS25160	73849..74265	-	417	WP_181502632.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV330_RS25165	74262..74492	-	231	WP_059362947.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV330_RS25170	75046..75396	+	351	WP_009654828.1	hypothetical protein	-
HV330_RS25175	75436..76203	+	768	WP_009654830.1	hypothetical protein	-
HV330_RS25180	76206..76982	+	777	WP_032426116.1	site-specific integrase	-
HV330_RS25185	77127..78116	-	990	WP_020804955.1	Rpn family recombination-promoting nuclease/putative transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..206642	206642

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15053.49 Da
• Isoelectric Point: 7.8637

>T163088 WP_181502632.1 NZ_CP056239:c74265-73849 [Citrobacter freundii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDAATDIKVALRLAGTPIGPNDTLIAGHAIAAGAVLVTRDGGEFERVPGLTLEDWAR

Nucleotide

• Length: 417 bp

>T163088 NZ_CP072326:3292587-3292937 [Aeromonas caviae]
ATGTGGACAGTAAAAACGACCGAGCTGTTTAATGCTTGGTTTGATGTGCAAGATGAAGCGACGCAGGAGAAAGTCTTGGC
TGGGCTACTCGCCTTGGCACAAGGTGGCCCATCGGTAGGGCGTCCCCTGGTCGATACCATCAAGGGCTCGCGTTTTACAA
ACTTAAAAGAGCTGAGGGTGCAGCATCAGGGAGAGCCGCTACGTGCATTCTTTGCCTTTGACCCGCTGCGACAAGCCATC
GTGCTTTGTGCTGGCAACAAGGGCGGGAACGAGAAGCGGTTTTACAAAGAGATGCTGCCGTTGGCTGAGGCACAGTACGC
CAGGCATCTGGCTGAGTTGGAGGAAAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8798.87 Da
• Isoelectric Point: 4.2917

>AT163088 WP_059362947.1 NZ_CP056239:c74492-74262 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFFELEKADPDFMAEREDVVTDEGRFEP

Nucleotide

• Length: 231 bp

>AT163088 NZ_CP072326:3292937-3293239 [Aeromonas caviae]
ATGGGAAGAACACTTGACGATTTGCTGGCCTCCCGTTCAGAAGAGAGTCGGCAACGCATCCAGGAAATGGCAACCGAGCT
GTTGCTGGAGGTGCGTATACAGGCGCTGCGCGAAGAGCTGGAGGTGTCGCAGGTTGAGTTGGCTAAGGCACTCGGTGTCA
GTCAGCCTGCCGTCGTGGCGATGGAGCAGCGTGGCAGTGATATCAAGCTCTCCACCATGAAACGCTATGTTGAAGCGATG
GGCGGCAAGCTGCGTGTAGATGTGGAATTGCCTACCGGCCGACACATCGGGTTCAACGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6S5K7T9