Detailed information of TA system TA163075

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 934341..935113
• Replicon: chromosome
• Accession: NZ_CP056238
• Organism: Citrobacter freundii strain RHBSTW-00935

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2I8S4E2
• Locus tag: HV330_RS04565
• Protein ID: WP_065805889.1
• Coordinates: 934724..935113 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV330_RS04560
• Protein ID: WP_171820947.1
• Coordinates: 934341..934667 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV330_RS04530	930786..931418	-	633	WP_072032909.1	inovirus Gp2 family protein	-
HV330_RS04535	931956..932780	+	825	WP_065805884.1	DUF945 domain-containing protein	-
HV330_RS04540	932777..933034	+	258	WP_044349282.1	DUF905 domain-containing protein	-
HV330_RS04545	933114..933557	+	444	WP_065805885.1	antirestriction protein	-
HV330_RS04550	933569..934048	+	480	WP_128301357.1	DNA repair protein RadC	-
HV330_RS04555	934062..934283	+	222	WP_053895773.1	DUF987 domain-containing protein	-
HV330_RS04560	934341..934667	+	327	WP_171820947.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV330_RS04565	934724..935113	+	390	WP_065805889.1	TA system toxin CbtA family protein	Toxin
HV330_RS04570	935200..936039	+	840	WP_128301359.1	DUF4942 domain-containing protein	-
HV330_RS04575	936777..936998	+	222	WP_003846405.1	hypothetical protein	-
HV330_RS04580	937035..937667	+	633	WP_003838326.1	hypothetical protein	-
HV330_RS04585	937751..938020	-	270	WP_064375540.1	hypothetical protein	-
HV330_RS04590	938096..938404	-	309	WP_170864030.1	hypothetical protein	-
HV330_RS04595	938386..938583	-	198	WP_044700790.1	hypothetical protein	-
HV330_RS04600	938677..939255	-	579	WP_061549908.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	905515..947183	41668

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14321.40 Da
• Isoelectric Point: 9.9883

>T163075 WP_065805889.1 NZ_CP056238:934724-935113 [Citrobacter freundii]
MQTKSSPLMRAASSRPSPVSVWQTLLTSLLAQHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T163075 NZ_CP072322:2032956-2033234 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12113.85 Da
• Isoelectric Point: 6.9554

>AT163075 WP_171820947.1 NZ_CP056238:934341-934667 [Citrobacter freundii]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANFNGQFCDADLRHLDQAFPVLMKQLELMLTSGELNPRHQHGIILYA
KGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT163075 NZ_CP072322:2032693-2032953 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A2I8S4E2