Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 299559..300145 Replicon chromosome
Accession NZ_CP056238
Organism Citrobacter freundii strain RHBSTW-00935

Toxin (Protein)


Gene name doc Uniprot ID A0A0J1QS94
Locus tag HV330_RS01420 Protein ID WP_032937237.1
Coordinates 299777..300145 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0J1N092
Locus tag HV330_RS01415 Protein ID WP_032937236.1
Coordinates 299559..299780 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV330_RS01390 295358..296284 + 927 WP_003023435.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HV330_RS01395 296281..297558 + 1278 WP_103848242.1 branched chain amino acid ABC transporter permease LivM -
HV330_RS01400 297555..298322 + 768 WP_003023438.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HV330_RS01405 298340..299053 + 714 WP_003827581.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
HV330_RS01410 299156..299437 + 282 WP_003023442.1 hypothetical protein -
HV330_RS01415 299559..299780 + 222 WP_032937236.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HV330_RS01420 299777..300145 + 369 WP_032937237.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HV330_RS01425 300389..301705 + 1317 WP_003023445.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
HV330_RS01430 301806..302693 + 888 WP_103848245.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
HV330_RS01435 302690..303535 + 846 WP_003023450.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
HV330_RS01440 303538..304608 + 1071 WP_003837845.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 261181..305348 44167


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13617.91 Da        Isoelectric Point: 6.9891

>T163072 WP_032937237.1 NZ_CP056238:299777-300145 [Citrobacter freundii]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIVLSANPDFVAMTVEAAAGQLTLEQIAHRLRH

Download         Length: 369 bp

>T163072 NZ_CP072322:968857-968964 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8306.41 Da        Isoelectric Point: 4.7906

>AT163072 WP_032937236.1 NZ_CP056238:299559-299780 [Citrobacter freundii]
MRTMNYSEARQNLAEVLESAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMIHGNEIRELADK

Download         Length: 222 bp

>AT163072 NZ_CP072322:c968804-968743 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1QS94

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1N092

References