Detailed information of TA system TA163070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 278956..279857
• Replicon: plasmid pRHBSTW-00965_2
• Accession: NZ_CP056233
• Organism: Citrobacter freundii strain RHBSTW-00965

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: HV336_RS26280
• Protein ID: WP_008786589.1
• Coordinates: 278956..279414 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HV336_RS26285
• Protein ID: WP_008786588.1
• Coordinates: 279411..279857 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV336_RS26235	274682..274924	+	243	WP_007372291.1	hypothetical protein	-
HV336_RS26240	274935..275333	+	399	WP_007372292.1	hypothetical protein	-
HV336_RS26245	275417..275743	+	327	WP_007372293.1	hypothetical protein	-
HV336_RS26250	276106..276444	-	339	WP_007372294.1	hypothetical protein	-
HV336_RS26255	276682..277125	+	444	Protein_336	hypothetical protein	-
HV336_RS26260	277244..277585	+	342	WP_007372296.1	hypothetical protein	-
HV336_RS26265	277744..278298	+	555	WP_007372297.1	hypothetical protein	-
HV336_RS26270	278348..278614	+	267	WP_007372298.1	hypothetical protein	-
HV336_RS26275	278682..278912	-	231	WP_007372299.1	hypothetical protein	-
HV336_RS26280	278956..279414	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
HV336_RS26285	279411..279857	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
HV336_RS26290	280006..280323	+	318	WP_016241555.1	hypothetical protein	-
HV336_RS26295	280365..281207	+	843	WP_007372303.1	hypothetical protein	-
HV336_RS26300	281179..281457	+	279	WP_007372304.1	hypothetical protein	-
HV336_RS26305	281517..281960	+	444	WP_007372305.1	hypothetical protein	-
HV336_RS26310	282247..282411	+	165	WP_007372306.1	hypothetical protein	-
HV336_RS26315	282979..283821	+	843	WP_181511243.1	HNH endonuclease	-
HV336_RS26320	284243..284494	+	252	WP_024196080.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..339929	339929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T163070 WP_008786589.1 NZ_CP056233:c279414-278956 [Citrobacter freundii]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T163070 NZ_CP072322:c766447-766271 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT163070 WP_008786588.1 NZ_CP056233:c279857-279411 [Citrobacter freundii]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT163070 NZ_CP072322:c766225-765809 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: S1EZ83