Detailed information of TA system TA163069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 271657..272207
• Replicon: plasmid pRHBSTW-00965_2
• Accession: NZ_CP056233
• Organism: Citrobacter freundii strain RHBSTW-00965

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: HV336_RS26210
• Protein ID: WP_007372286.1
• Coordinates: 271899..272207 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: HV336_RS26205
• Protein ID: WP_007372285.1
• Coordinates: 271657..271896 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV336_RS26155	266664..267059	+	396	WP_024196081.1	hypothetical protein	-
HV336_RS26160	267241..267432	+	192	WP_016241560.1	hypothetical protein	-
HV336_RS26165	267563..268258	+	696	WP_007372278.1	hypothetical protein	-
HV336_RS26170	268281..268478	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
HV336_RS26175	268531..268989	+	459	WP_008786599.1	hypothetical protein	-
HV336_RS26180	269137..269277	+	141	WP_007372281.1	hypothetical protein	-
HV336_RS26185	269293..269925	+	633	WP_007372282.1	hypothetical protein	-
HV336_RS26190	269922..270332	+	411	WP_016241559.1	hypothetical protein	-
HV336_RS26195	270386..271057	+	672	WP_007372283.1	hypothetical protein	-
HV336_RS26200	271054..271518	+	465	WP_008786596.1	hypothetical protein	-
HV336_RS26205	271657..271896	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HV336_RS26210	271899..272207	+	309	WP_007372286.1	CcdB family protein	Toxin
HV336_RS26215	272216..272386	+	171	WP_007372287.1	hypothetical protein	-
HV336_RS26220	272541..273095	+	555	WP_007372288.1	hypothetical protein	-
HV336_RS26225	273165..273782	+	618	WP_007372289.1	hypothetical protein	-
HV336_RS26230	273865..274335	+	471	WP_007372290.1	hypothetical protein	-
HV336_RS26235	274682..274924	+	243	WP_007372291.1	hypothetical protein	-
HV336_RS26240	274935..275333	+	399	WP_007372292.1	hypothetical protein	-
HV336_RS26245	275417..275743	+	327	WP_007372293.1	hypothetical protein	-
HV336_RS26250	276106..276444	-	339	WP_007372294.1	hypothetical protein	-
HV336_RS26255	276682..277125	+	444	Protein_336	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..339929	339929

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T163069 WP_007372286.1 NZ_CP056233:271899-272207 [Citrobacter freundii]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T163069 NZ_CP072322:321123-321226 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT163069 WP_007372285.1 NZ_CP056233:271657-271896 [Citrobacter freundii]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT163069 NZ_CP072322:c321264-320993 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3