Detailed information of TA system TA163068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 82345..82922
• Replicon: plasmid pRHBSTW-00965_2
• Accession: NZ_CP056233
• Organism: Citrobacter freundii strain RHBSTW-00965

Toxin (Protein)

• Gene name: parE
• Uniprot ID: I3W245
• Locus tag: HV336_RS25025
• Protein ID: WP_015059150.1
• Coordinates: 82345..82656 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3DB50
• Locus tag: HV336_RS25030
• Protein ID: WP_014542884.1
• Coordinates: 82644..82922 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV336_RS25005	77600..78025	+	426	WP_000065758.1	glutaredoxin-dependent arsenate reductase	-
HV336_RS25010	78056..78460	-	405	WP_004118313.1	arsenic transporter ATPase	-
HV336_RS25015	78469..79041	-	573	WP_001556711.1	recombinase family protein	-
HV336_RS25020	79205..82213	+	3009	WP_012817690.1	Tn3 family transposase	-
HV336_RS25025	82345..82656	-	312	WP_015059150.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV336_RS25030	82644..82922	-	279	WP_014542884.1	CopG family ribbon-helix-helix protein	Antitoxin
HV336_RS25035	83597..84091	-	495	WP_021243016.1	peptide-methionine (S)-S-oxide reductase MsrA	-
HV336_RS25040	84081..84542	-	462	WP_021243015.1	peptide-methionine (R)-S-oxide reductase MsrB	-
HV336_RS25045	84566..84703	-	138	WP_021243014.1	hypothetical protein	-
HV336_RS25050	84777..85283	-	507	WP_001567380.1	DUF417 family protein	-
HV336_RS25055	85314..85574	-	261	WP_001567381.1	DUF1471 domain-containing protein	-
HV336_RS25060	85787..86344	-	558	WP_001567382.1	TetR/AcrR family transcriptional regulator	-
HV336_RS25065	86441..86707	-	267	WP_001567383.1	DUF1471 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..339929	339929
	-	inside	IScluster/Tn	-	-	78469..91893	13424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11840.74 Da
• Isoelectric Point: 9.8620

>T163068 WP_015059150.1 NZ_CP056233:c82656-82345 [Citrobacter freundii]
MPQVIVSALAQRDLQRLQDFLKTKNRLAARKAGEVIIRAIQQLKTQPDMGRPVSLLPLEYQELVIGFGDSGYVMLYRHDR
ETDRIVILTVRHQKEAGYHGLQE

Nucleotide

• Length: 312 bp

>T163068 NZ_CP072322:c130039-129665 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10721.74 Da
• Isoelectric Point: 4.6216

>AT163068 WP_014542884.1 NZ_CP056233:c82922-82644 [Citrobacter freundii]
MSAATSIRLDDELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLSAEEVEAWIDTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT163068 NZ_CP072322:c130496-130128 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A4P6WJV0

Antitoxin

• AlphaFold DB: E3DB50