Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 36146..36949 Replicon plasmid pRHBSTW-00965_2
Accession NZ_CP056233
Organism Citrobacter freundii strain RHBSTW-00965

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag HV336_RS24750 Protein ID WP_048213819.1
Coordinates 36146..36676 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag HV336_RS24755 Protein ID WP_048213818.1
Coordinates 36680..36949 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV336_RS24730 32138..32671 + 534 WP_048213822.1 hypothetical protein -
HV336_RS24735 33026..34048 + 1023 WP_048213854.1 adenylate/guanylate cyclase domain-containing protein -
HV336_RS24740 34807..35052 + 246 WP_048213821.1 hypothetical protein -
HV336_RS24745 35057..36061 + 1005 WP_048213820.1 hypothetical protein -
HV336_RS24750 36146..36676 - 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
HV336_RS24755 36680..36949 - 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
HV336_RS24760 37164..37661 - 498 Protein_37 IS3 family transposase -
HV336_RS24765 37742..39241 - 1500 WP_048213817.1 group II intron reverse transcriptase/maturase -
HV336_RS24770 39914..40599 - 686 Protein_39 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..339929 339929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T163067 WP_048213819.1 NZ_CP056233:c36676-36146 [Citrobacter freundii]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

>T163067 NZ_CP072316:30990-31238 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT163067 WP_048213818.1 NZ_CP056233:c36949-36680 [Citrobacter freundii]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT163067 NZ_CP072316:31235-31582 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References