Detailed information of TA system TA163066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4966817..4967433
• Replicon: chromosome
• Accession: NZ_CP056232
• Organism: Citrobacter freundii strain RHBSTW-00965

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HV336_RS23655
• Protein ID: WP_181509822.1
• Coordinates: 4966817..4967191 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7I7DRJ8
• Locus tag: HV336_RS23660
• Protein ID: WP_003825279.1
• Coordinates: 4967191..4967433 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV336_RS23640	4964320..4965222	+	903	WP_042313505.1	formate dehydrogenase O subunit beta	-
HV336_RS23645	4965219..4965854	+	636	WP_181509821.1	formate dehydrogenase cytochrome b556 subunit	-
HV336_RS23650	4965851..4966780	+	930	WP_043018887.1	formate dehydrogenase accessory protein FdhE	-
HV336_RS23655	4966817..4967191	-	375	WP_181509822.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV336_RS23660	4967191..4967433	-	243	WP_003825279.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV336_RS23665	4967639..4968547	+	909	WP_181509823.1	alpha/beta hydrolase	-
HV336_RS23670	4968612..4968854	+	243	WP_003825282.1	type II toxin-antitoxin system ParD family antitoxin	-
HV336_RS23675	4968847..4969134	+	288	WP_181509824.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV336_RS23680	4969140..4970081	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HV336_RS23685	4970126..4970563	-	438	WP_003825289.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV336_RS23690	4970560..4971432	-	873	WP_181509825.1	virulence factor BrkB family protein	-
HV336_RS23695	4971426..4972025	-	600	WP_008786997.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13638.82 Da
• Isoelectric Point: 9.6927

>T163066 WP_181509822.1 NZ_CP056232:c4967191-4966817 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWSPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEHGMKLPDAIILATAQIHRLTLVTRNTKDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T163066 NZ_CP072316:29026-29277 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9421.72 Da
• Isoelectric Point: 4.3437

>AT163066 WP_003825279.1 NZ_CP056232:c4967433-4967191 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETAISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT163066 NZ_CP072316:29264-29491 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7DRJ8