Detailed information of TA system TA163065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4798006..4798672
• Replicon: chromosome
• Accession: NZ_CP056232
• Organism: Citrobacter freundii strain RHBSTW-00965

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A6N6K5Y9
• Locus tag: HV336_RS22885
• Protein ID: WP_005132818.1
• Coordinates: 4798313..4798672 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: HV336_RS22880
• Protein ID: WP_181509756.1
• Coordinates: 4798006..4798323 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV336_RS22850	4793595..4794407	+	813	WP_181509751.1	shikimate 5-dehydrogenase	-
HV336_RS22855	4794457..4794813	-	357	WP_181511230.1	hypothetical protein	-
HV336_RS22860	4794955..4795116	+	162	WP_181509752.1	hypothetical protein	-
HV336_RS22865	4795316..4795921	+	606	WP_181509753.1	hypothetical protein	-
HV336_RS22870	4796362..4796997	-	636	WP_181509754.1	transglycosylase domain-containing protein	-
HV336_RS22875	4796976..4797524	-	549	WP_181509755.1	hypothetical protein	-
HV336_RS22880	4798006..4798323	-	318	WP_181509756.1	helix-turn-helix domain-containing protein	Antitoxin
HV336_RS22885	4798313..4798672	-	360	WP_005132818.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV336_RS22890	4798886..4799575	+	690	WP_048236375.1	dipeptidase PepE	-
HV336_RS22895	4799745..4801373	-	1629	WP_172743590.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13476.49 Da
• Isoelectric Point: 10.3799

>T163065 WP_005132818.1 NZ_CP056232:c4798672-4798313 [Citrobacter freundii]
MTKPLYWVGHARKDLQGMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNTWRAVYTIQLKNAVYVL
HVFQKKSVSGKATPKPEINLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T163065 NZ_CP072315:146270-147595 [Escherichia coli]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11388.16 Da
• Isoelectric Point: 5.0787

>AT163065 WP_181509756.1 NZ_CP056232:c4798323-4798006 [Citrobacter freundii]
MSHDVETSSGNVYADLGEYDAEQMQLKAQLAMVIGEILQCKGLTQQQAAQMLGMTQPKLSNLLRGQFRGISEAKMLECLV
KLGRDVEIVVGKEGNVQGSLKVSFG

Nucleotide

• Length: 318 bp

>AT163065 NZ_CP072315:145972-146265 [Escherichia coli]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Structures

Toxin

• AlphaFold DB: A0A6N6K5Y9