Detailed information of TA system TA163064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4705897..4706432
• Replicon: chromosome
• Accession: NZ_CP056232
• Organism: Citrobacter freundii strain RHBSTW-00965

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HV336_RS22440
• Protein ID: WP_085049650.1
• Coordinates: 4706145..4706432 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D4BKM9
• Locus tag: HV336_RS22435
• Protein ID: WP_006688247.1
• Coordinates: 4705897..4706148 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV336_RS22405	4701374..4702132	+	759	WP_003826710.1	phosphonate C-P lyase system protein PhnK	-
HV336_RS22410	4702254..4702940	+	687	WP_137382890.1	phosphonate C-P lyase system protein PhnL	-
HV336_RS22415	4702937..4704073	+	1137	WP_181509710.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
HV336_RS22420	4704076..4704630	+	555	WP_149281892.1	ribose 1,5-bisphosphokinase	-
HV336_RS22425	4704617..4705051	+	435	WP_181509711.1	aminoalkylphosphonate N-acetyltransferase	-
HV336_RS22430	4705060..4705818	+	759	WP_181509712.1	phosphonate metabolism protein PhnP	-
HV336_RS22435	4705897..4706148	+	252	WP_006688247.1	plasmid stabilization protein	Antitoxin
HV336_RS22440	4706145..4706432	+	288	WP_085049650.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV336_RS22445	4706429..4706758	-	330	WP_181509713.1	hypothetical protein	-
HV336_RS22450	4706828..4709092	-	2265	WP_181509714.1	hybrid sensor histidine kinase/response regulator	-
HV336_RS22455	4709207..4710730	+	1524	WP_181509715.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11089.04 Da
• Isoelectric Point: 10.3527

>T163064 WP_085049650.1 NZ_CP056232:4706145-4706432 [Citrobacter freundii]
MSYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCENPHVPSAKLRGIKDCYKIKLRTSGFRLVYQVIDDTLVIAVVAVGK
RERSEVYNLASERLR

Nucleotide

• Length: 288 bp

>T163064 NZ_CP072314:c42619-42137 [Escherichia coli]
ATGGAAATAAATGTCACCGCGCCGGCATTGCTGACAGATGAGCATATACTTCAGCCATTTGACTGTGGGAATGAGGTGTT
AAGTAACTGGTTGCGTGGGCGGGCTATGAAAAACCAGATACTCAATGCTTCCCGAACGTTCGTCATTTGTCTGGAGGATA
CTTTACGTGTTGTGGGGTATTACTCATTAGCTACTGGTTCTGTCACACATGCCGAGCTTGGGCGCAGTTTGCGCCATAAT
ATGCCCAATCCTGTACCTGTTGTTCTTTTAGGGCGTCTGGCTGTCGATGTTTGTACTCAGGGACATGGTTTCGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGGTTGCCAATCTGGCTGAACAGGTTGGCATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGACGCCAGAGTTTTTTATGAGCATTTTGGTTTTGTTCAGTCAGTTGTGGCACCTGAGACCTTGTTTTATAAAATT
TGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9215.53 Da
• Isoelectric Point: 4.2965

>AT163064 WP_006688247.1 NZ_CP056232:4705897-4706148 [Citrobacter freundii]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQQLVKLVHERSQQPLVDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT163064 NZ_CP072314:c42912-42610 [Escherichia coli]
ATGGAGGTTGTTATGCCTACTACTGCAAACATCCGTAAAACACCGCGAGAAAATCAGATTAACATCCGGGCAACGGATGA
GGAACGAGCCGTTATTGATTATGCCGCTAGCCTGGTCAATAAGAACCGTACTGATTTCATCATTGAACTGGCTTACCAGG
AAGCTAAAAACATCATCTTGGATCAGCGTTTATTTGTGCTGGATGATGAATGTTATGATTCATTTATTACGCAACTGGAA
GCTCCTGTACAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7X1BT32