Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4096140..4096819 Replicon chromosome
Accession NZ_CP056232
Organism Citrobacter freundii strain RHBSTW-00965

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag HV336_RS19610 Protein ID WP_003031349.1
Coordinates 4096478..4096819 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag HV336_RS19605 Protein ID WP_003031347.1
Coordinates 4096140..4096457 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV336_RS19580 4092233..4094230 - 1998 WP_032939120.1 choline BCCT transporter BetT -
HV336_RS19585 4094734..4094925 + 192 Protein_3845 DUF905 family protein -
HV336_RS19590 4094939..4095400 + 462 WP_003031344.1 antirestriction protein -
HV336_RS19595 4095416..4095892 + 477 WP_003031345.1 RadC family protein -
HV336_RS19600 4095901..4096122 + 222 WP_003031346.1 DUF987 domain-containing protein -
HV336_RS19605 4096140..4096457 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV336_RS19610 4096478..4096819 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
HV336_RS19620 4097399..4098652 - 1254 WP_181509437.1 glutamate-5-semialdehyde dehydrogenase -
HV336_RS19625 4098664..4099767 - 1104 WP_003031373.1 glutamate 5-kinase -
HV336_RS19630 4100057..4101112 + 1056 WP_003830831.1 phosphoporin PhoE -
HV336_RS19635 4101301..4101702 - 402 WP_003031376.1 sigma factor-binding protein Crl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T163063 WP_003031349.1 NZ_CP056232:4096478-4096819 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T163063 NZ_CP072313:c3364-3110 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAATGATTGATGAAAAATATCATTTTGTAAAAAAAATAAGCAG
CTATTCAAAGGTGTACACAGAAGGAAAAACTAAAACGACAAAGAGAACGTTAAGCGAACTGGTTTTTAATGAAAATGAAG
TTGGGGCAATAGATTTTACAAAAAATGGTTTAAAACCTGTTGATAAGAGTATTCTTTTAACCATGATGAAAGAATATAAG
GAGAATGAAACATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT163063 WP_003031347.1 NZ_CP056232:4096140-4096457 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT163063 NZ_CP072313:c3113-2856 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAATGCGGTGAAAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAGGAAGCAAAAACCCACAACCAATTAAATTTGTCAAAAGGTGAATTTG
AGTATCTGTTATTGCTTGCTGGAGAACACCCTGAATACGTCCTTTGTAAAAGGGGCAACATGACGCACAACGAAATCAAT
GAGCAATTGCGCCCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References