163062

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3859457..3860077 Replicon chromosome
Accession NZ_CP056232
Organism Citrobacter freundii strain RHBSTW-00965

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV336_RS18480 Protein ID WP_002892050.1
Coordinates 3859859..3860077 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV336_RS18475 Protein ID WP_003021733.1
Coordinates 3859457..3859831 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV336_RS18465 3854602..3855795 + 1194 WP_003831044.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV336_RS18470 3855818..3858967 + 3150 WP_126319003.1 efflux RND transporter permease AcrB -
HV336_RS18475 3859457..3859831 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV336_RS18480 3859859..3860077 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV336_RS18485 3860259..3860810 + 552 WP_003835924.1 maltose O-acetyltransferase -
HV336_RS18490 3860927..3861397 + 471 WP_020996574.1 YlaC family protein -
HV336_RS18495 3861476..3861616 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV336_RS18500 3861618..3861878 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
HV336_RS18505 3862067..3863620 + 1554 WP_181509316.1 EAL domain-containing protein -
HV336_RS18510 3863672..3864025 - 354 WP_003831033.1 DUF1428 family protein -
HV336_RS18515 3864090..3864719 - 630 WP_181509317.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163062 WP_002892050.1 NZ_CP056232:3859859-3860077 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163062 NZ_CP072312:60464-60751 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163062 WP_003021733.1 NZ_CP056232:3859457-3859831 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163062 NZ_CP072312:60216-60467 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTTTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References