Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2603051..2603787 Replicon chromosome
Accession NZ_CP056232
Organism Citrobacter freundii strain RHBSTW-00965

Toxin (Protein)


Gene name tacT Uniprot ID -
Locus tag HV336_RS12380 Protein ID WP_181508537.1
Coordinates 2603305..2603787 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag HV336_RS12375 Protein ID WP_008784769.1
Coordinates 2603051..2603317 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV336_RS12355 2598100..2599287 + 1188 WP_181508533.1 outer membrane protein transport protein -
HV336_RS12360 2599528..2600157 + 630 WP_181508534.1 winged helix-turn-helix transcriptional regulator -
HV336_RS12365 2600273..2600659 - 387 WP_181508535.1 hypothetical protein -
HV336_RS12370 2600915..2602897 + 1983 WP_181508536.1 MBL fold metallo-hydrolase -
HV336_RS12375 2603051..2603317 + 267 WP_008784769.1 DUF1778 domain-containing protein Antitoxin
HV336_RS12380 2603305..2603787 + 483 WP_181508537.1 GNAT family N-acetyltransferase Toxin
HV336_RS12385 2604868..2605110 + 243 Protein_2428 transposase -
HV336_RS12390 2605348..2605803 + 456 WP_181508538.1 hypothetical protein -
HV336_RS12395 2605797..2606708 + 912 WP_181508539.1 hypothetical protein -
HV336_RS12400 2606882..2607182 + 301 Protein_2431 transposase -
HV336_RS12405 2607524..2608318 - 795 WP_181508540.1 hypothetical protein -
HV336_RS12410 2608579..2608734 + 156 Protein_2433 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2599528..2630242 30714
- flank IS/Tn - - 2608579..2608785 206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17586.38 Da        Isoelectric Point: 9.5814

>T163061 WP_181508537.1 NZ_CP056232:2603305-2603787 [Citrobacter freundii]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRSM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHALTDEAKRFYLHHGFKASQLQERTLFLRLS

Download         Length: 483 bp

>T163061 NZ_CP072312:c7317-7039 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10159.65 Da        Isoelectric Point: 4.6558

>AT163061 WP_008784769.1 NZ_CP056232:2603051-2603317 [Citrobacter freundii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Download         Length: 267 bp

>AT163061 NZ_CP072312:c7586-7317 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References