Detailed information of TA system TA163047

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4117134..4117813
• Replicon: chromosome
• Accession: NZ_CP056229
• Organism: Citrobacter freundii strain RHBSTW-00968

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV337_RS19505
• Protein ID: WP_032666240.1
• Coordinates: 4117134..4117475 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV337_RS19510
• Protein ID: WP_181490642.1
• Coordinates: 4117496..4117813 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV337_RS19485	4113162..4113416	+	255	WP_181490638.1	(2Fe-2S)-binding protein	-
HV337_RS19490	4113403..4114659	+	1257	WP_181490639.1	NAD(P)/FAD-dependent oxidoreductase	-
HV337_RS19495	4115155..4115952	-	798	WP_181490640.1	helix-turn-helix transcriptional regulator	-
HV337_RS19500	4116185..4117018	-	834	WP_181490641.1	DUF4942 domain-containing protein	-
HV337_RS19505	4117134..4117475	-	342	WP_032666240.1	TA system toxin CbtA family protein	Toxin
HV337_RS19510	4117496..4117813	-	318	WP_181490642.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV337_RS19515	4117831..4118052	-	222	WP_181490643.1	DUF987 domain-containing protein	-
HV337_RS19520	4118061..4118543	-	483	WP_181490644.1	DNA repair protein RadC	-
HV337_RS19525	4118552..4119013	-	462	WP_181490645.1	antirestriction protein	-
HV337_RS19530	4119115..4119354	-	240	WP_063844625.1	DUF905 domain-containing protein	-
HV337_RS19535	4119431..4119898	-	468	WP_181490646.1	hypothetical protein	-
HV337_RS19540	4119922..4120365	-	444	WP_181490647.1	hypothetical protein	-
HV337_RS19545	4120365..4120601	-	237	WP_063618751.1	hypothetical protein	-
HV337_RS19550	4120642..4121343	-	702	WP_181490648.1	WYL domain-containing protein	-
HV337_RS19555	4121560..4122381	-	822	WP_181490649.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4116185..4141560	25375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12838.76 Da
• Isoelectric Point: 9.9971

>T163047 WP_032666240.1 NZ_CP056229:c4117475-4117134 [Citrobacter freundii]
MKPQSATTSRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFSDETVIQEHINAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNAVR

Nucleotide

• Length: 342 bp

>T163047 NZ_CP072303:2754-3110 [Acinetobacter baumannii]
ATGTGGACAGTCATTACGACAGATCTATTTAATGAATGGCTTGAACAGCAAGATGAATCAACACAAGAGAAGGTTTTAGC
TGCTTTGGTTGTTCTGCAACAACAGGGACCGAGCTTAGGCCGCCCTTTAGTAGATACCGTGTATGACTCTAAATTTACCA
ATATGAAAGAGCTTCGTGTTCAACATCGTGGTAAACCACTCCGAGCTTTTTTTGCATTTGATCCATTACGACAGGCCATT
GTGCTGTGTATTGGTGATAAAGGTGGTAAAAAGCGATTCTATACAGAGATGCTGGCGATTGCAGATCAACAATATGAACT
TCATCTCTCAACATTAGGAGAACAATCTAATGGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11838.41 Da
• Isoelectric Point: 6.8332

>AT163047 WP_181490642.1 NZ_CP056229:c4117813-4117496 [Citrobacter freundii]
MSKTTWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDSECRKLDETFPHFISQMESMLTTGELNPQYAHCVTLCH
SGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT163047 NZ_CP072303:3103-3405 [Acinetobacter baumannii]
ATGGCTAAAACTTTGCAAGAATTATTAGCTAACCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATAGCTGATGAATT
GCTATTAGAAAATCAGCTTTACCTTATTCGTGAAGAACTCGAAATCTCTCAAAAAGAACTGGCTGAAACTTTGGGAATAA
AACAGCCATCACTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATCTCAACCATGAAAAAGTATGTTGAAGCAATG
GGAGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA