Detailed information of TA system TA163046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4078848..4079424
• Replicon: chromosome
• Accession: NZ_CP056229
• Organism: Citrobacter freundii strain RHBSTW-00968

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HV337_RS19335
• Protein ID: WP_181490627.1
• Coordinates: 4079137..4079424 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HV337_RS19330
• Protein ID: WP_117342368.1
• Coordinates: 4078848..4079150 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV337_RS19315	4075316..4077466	+	2151	WP_135912629.1	pyruvate/proton symporter BtsT	-
HV337_RS19320	4077574..4077768	+	195	WP_181490625.1	YbdD/YjiX family protein	-
HV337_RS19325	4077781..4078737	+	957	WP_181490626.1	GTPase	-
HV337_RS19330	4078848..4079150	-	303	WP_117342368.1	BrnA antitoxin family protein	Antitoxin
HV337_RS19335	4079137..4079424	-	288	WP_181490627.1	BrnT family toxin	Toxin
HV337_RS19340	4079597..4079761	-	165	WP_117342370.1	DUF1127 domain-containing protein	-
HV337_RS19345	4079938..4081350	+	1413	WP_181490628.1	PLP-dependent aminotransferase family protein	-
HV337_RS19350	4081377..4081973	-	597	Protein_3786	Rpn family recombination-promoting nuclease/putative transposase	-
HV337_RS19355	4082420..4083661	+	1242	WP_135912631.1	multidrug efflux MFS transporter MdtM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11392.77 Da
• Isoelectric Point: 7.5239

>T163046 WP_181490627.1 NZ_CP056229:c4079424-4079137 [Citrobacter freundii]
MPMEFEWDANKAQSNHRKHGVRFEDAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIVVILVTHSVRFESGFEVIRIIS
ARKADRKERNRYEHD

Nucleotide

• Length: 288 bp

>T163046 NZ_CP072302:3823-4182 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11444.95 Da
• Isoelectric Point: 8.5366

>AT163046 WP_117342368.1 NZ_CP056229:c4079150-4078848 [Citrobacter freundii]
MSMIKHKRGSASALSAQHEAELKALANKPDDDIDYSDIPVTEDEQWSEANRGTFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT163046 NZ_CP072302:4182-4481 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA