Detailed information of TA system TA163045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3553866..3554486
• Replicon: chromosome
• Accession: NZ_CP056229
• Organism: Citrobacter freundii strain RHBSTW-00968

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HV337_RS16895
• Protein ID: WP_002892050.1
• Coordinates: 3554268..3554486 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV337_RS16890
• Protein ID: WP_115259201.1
• Coordinates: 3553866..3554240 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV337_RS16880	3549012..3550205	+	1194	WP_096758038.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV337_RS16885	3550228..3553377	+	3150	WP_096758039.1	efflux RND transporter permease AcrB	-
HV337_RS16890	3553866..3554240	+	375	WP_115259201.1	Hha toxicity modulator TomB	Antitoxin
HV337_RS16895	3554268..3554486	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HV337_RS16900	3554868..3555341	+	474	WP_096758041.1	YlaC family protein	-
HV337_RS16905	3555415..3555555	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
HV337_RS16910	3555557..3555817	-	261	WP_115259215.1	type B 50S ribosomal protein L31	-
HV337_RS16915	3556002..3557555	+	1554	WP_115259216.1	EAL domain-containing protein	-
HV337_RS16920	3557609..3557962	-	354	WP_115259217.1	DUF1428 family protein	-
HV337_RS16925	3558045..3558656	-	612	WP_153879388.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T163045 WP_002892050.1 NZ_CP056229:3554268-3554486 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T163045 NZ_CP072300:c2955635-2955453 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14454.26 Da
• Isoelectric Point: 5.5653

>AT163045 WP_115259201.1 NZ_CP056229:3553866-3554240 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT163045 NZ_CP072300:c2955360-2954956 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2