Detailed information of TA system TA163030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 45532..46057
• Replicon: plasmid pRHBSTW-00973_4
• Accession: NZ_CP056225
• Organism: Klebsiella pneumoniae strain RHBSTW-00973

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: HV338_RS28150
• Protein ID: WP_016946795.1
• Coordinates: 45752..46057 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: HV338_RS28145
• Protein ID: WP_004197642.1
• Coordinates: 45532..45750 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV338_RS28135	43214..44242	-	1029	WP_001568023.1	Abi family protein	-
HV338_RS28140	44383..44952	-	570	WP_016162066.1	hypothetical protein	-
HV338_RS28145	45532..45750	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV338_RS28150	45752..46057	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV338_RS28155	46215..46724	+	510	WP_052522797.1	hypothetical protein	-
HV338_RS28160	46903..47679	+	777	WP_049245386.1	site-specific integrase	-
HV338_RS28165	47737..47994	-	258	WP_000764642.1	hypothetical protein	-
HV338_RS28170	48123..48227	-	105	WP_032409716.1	hypothetical protein	-
HV338_RS28175	48762..49628	+	867	WP_004118283.1	replication initiation protein	-
HV338_RS28180	49913..50182	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..69970	69970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T163030 WP_016946795.1 NZ_CP056225:45752-46057 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T163030 NZ_CP072285:c3204599-3204417 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT163030 WP_004197642.1 NZ_CP056225:45532-45750 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT163030 NZ_CP072285:c3204324-3203920 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG