Detailed information of TA system TA163028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 56294..57030
• Replicon: plasmid pRHBSTW-00973_2
• Accession: NZ_CP056223
• Organism: Klebsiella pneumoniae strain RHBSTW-00973

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV338_RS26785
• Protein ID: WP_181479570.1
• Coordinates: 56294..56776 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: HV338_RS26790
• Protein ID: WP_049083184.1
• Coordinates: 56764..57030 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV338_RS26760	51315..51743	-	429	WP_001554382.1	GFA family protein	-
HV338_RS26765	52002..52970	+	969	WP_000654811.1	IS5 family transposase	-
HV338_RS26770	53015..53608	-	594	WP_181479569.1	helix-turn-helix domain-containing protein	-
HV338_RS26775	53873..54577	-	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
HV338_RS26780	54737..56083	-	1347	WP_077251107.1	ISNCY family transposase	-
HV338_RS26785	56294..56776	-	483	WP_181479570.1	GNAT family N-acetyltransferase	Toxin
HV338_RS26790	56764..57030	-	267	WP_049083184.1	DUF1778 domain-containing protein	Antitoxin
HV338_RS26795	57126..57449	+	324	WP_115656969.1	hypothetical protein	-
HV338_RS26800	57502..58283	-	782	Protein_68	AAA family ATPase	-
HV338_RS26805	58280..59302	-	1023	WP_110184588.1	IS21 family transposase	-
HV338_RS26810	59392..59856	+	465	WP_177957707.1	hypothetical protein	-
HV338_RS26815	59850..60547	-	698	Protein_71	IS1 family transposase	-
HV338_RS26820	60763..61731	-	969	WP_181479571.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..176327	176327
	-	inside	IScluster/Tn	-	-	48723..61731	13008

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17354.96 Da
• Isoelectric Point: 9.1188

>T163028 WP_181479570.1 NZ_CP056223:c56776-56294 [Klebsiella pneumoniae]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVVENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T163028 NZ_CP072285:709379-709768 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCATTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAGTAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10117.61 Da
• Isoelectric Point: 4.6437

>AT163028 WP_049083184.1 NZ_CP056223:c57030-56764 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDVQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT163028 NZ_CP072285:709115-709372 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA