Detailed information of TA system TA163027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 23490..24012
• Replicon: plasmid pRHBSTW-00973_2
• Accession: NZ_CP056223
• Organism: Klebsiella pneumoniae strain RHBSTW-00973

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: HV338_RS26610
• Protein ID: WP_004187019.1
• Coordinates: 23490..23774 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: HV338_RS26615
• Protein ID: WP_004187025.1
• Coordinates: 23764..24012 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV338_RS26585	19499..20071	+	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
HV338_RS26590	20271..21194	+	924	WP_000167917.1	cation diffusion facilitator family transporter	-
HV338_RS26595	21457..21696	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	-
HV338_RS26600	21696..21983	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	-
HV338_RS26605	22131..23474	+	1344	WP_040120121.1	ISNCY family transposase	-
HV338_RS26610	23490..23774	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV338_RS26615	23764..24012	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
HV338_RS26620	24521..25489	-	969	WP_181479567.1	IS5 family transposase	-
HV338_RS26625	25524..25796	+	273	Protein_33	transposase domain-containing protein	-
HV338_RS26630	25918..27651	-	1734	WP_032738458.1	amidohydrolase	-
HV338_RS26635	27702..28343	-	642	WP_032430981.1	ANTAR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..176327	176327

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T163027 WP_004187019.1 NZ_CP056223:c23774-23490 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T163027 NZ_CP072283:2754-3110 [Acinetobacter baumannii]
ATGTGGACAGTCATTACGACAGATCTATTTAATGAATGGCTTGAACAGCAAGATGAATCAACACAAGAGAAGGTTTTAGC
TGCTTTGGTTGTTCTGCAACAACAGGGACCGAGCTTAGGCCGCCCTTTAGTAGATACCGTGTATGACTCTAAATTTACCA
ATATGAAAGAGCTTCGTGTTCAACATCGTGGTAAACCACTCCGAGCTTTTTTTGCATTTGATCCATTACGACAGGCCATT
GTGCTGTGTATTGGTGATAAAGGTGGTAAAAAGCGATTCTATACAGAGATGCTGGCGATTGCAGATCAACAATATGAACT
TCATCTCTCAACATTAGGAGAACAATCTAATGGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT163027 WP_004187025.1 NZ_CP056223:c24012-23764 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT163027 NZ_CP072283:3103-3405 [Acinetobacter baumannii]
ATGGCTAAAACTTTGCAAGAATTATTAGCTAACCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATAGCTGATGAATT
GCTATTAGAAAATCAGCTTTACCTTATTCGTGAAGAACTCGAAATCTCTCAAAAAGAACTGGCTGAAACTTTGGGAATAA
AACAGCCATCACTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATCTCAACCATGAAAAAGTATGTTGAAGCAATG
GGAGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4