Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5318452..5319077 Replicon chromosome
Accession NZ_CP056222
Organism Klebsiella pneumoniae strain RHBSTW-00973

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HV338_RS26095 Protein ID WP_002882817.1
Coordinates 5318452..5318835 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV338_RS26100 Protein ID WP_004150355.1
Coordinates 5318835..5319077 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV338_RS26080 5315818..5316720 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV338_RS26085 5316717..5317352 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HV338_RS26090 5317349..5318278 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV338_RS26095 5318452..5318835 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HV338_RS26100 5318835..5319077 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV338_RS26105 5319282..5320199 + 918 WP_004181612.1 alpha/beta hydrolase -
HV338_RS26110 5320213..5321154 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV338_RS26115 5321199..5321636 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV338_RS26120 5321633..5322493 - 861 WP_004146232.1 virulence factor BrkB family protein -
HV338_RS26125 5322487..5323086 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T163025 WP_002882817.1 NZ_CP056222:c5318835-5318452 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T163025 NZ_CP072280:c2956823-2956641 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT163025 WP_004150355.1 NZ_CP056222:c5319077-5318835 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT163025 NZ_CP072280:c2956548-2956144 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References