Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5149459..5150084 Replicon chromosome
Accession NZ_CP056216
Organism Klebsiella pneumoniae strain RHBSTW-00982

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag HV343_RS25075 Protein ID WP_004187928.1
Coordinates 5149459..5149842 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV343_RS25080 Protein ID WP_004150355.1
Coordinates 5149842..5150084 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV343_RS25060 5146825..5147727 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV343_RS25065 5147724..5148359 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HV343_RS25070 5148356..5149285 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV343_RS25075 5149459..5149842 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
HV343_RS25080 5149842..5150084 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV343_RS25085 5150278..5151195 + 918 WP_004187929.1 alpha/beta hydrolase -
HV343_RS25090 5151209..5152150 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV343_RS25095 5152195..5152632 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV343_RS25100 5152629..5153489 - 861 WP_004146232.1 virulence factor BrkB family protein -
HV343_RS25105 5153483..5154082 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T163007 WP_004187928.1 NZ_CP056216:c5149842-5149459 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T163007 NZ_CP072265:5435394-5435810 [Xanthomonas citri pv. glycines]
GTGATCCTGCTCGATACCAATGTCATCTCCGAACTTTGGCGGCCTCAGCCTAATCCGCAAGTCGTCGCCTGGATCGATGC
ACAAGCTGTCGAGACACTGTTCCTGTCGGTCGTGACGGTGGCTGAACTCCGTTTCGGTATTGCCGTGATGCCCAAGGGCC
GAAAGCGATCCACCTTGCATGCTCGGTTGGAGGGCGAGGTATTGCCACTGTTCGACGGGCGCCTGCTCGCATTCGATCTG
GATGCATCACACGCATTTGCGGCGCTTGCATCGAAAGCCAGAACCGCAGGACTCACACTCGGGCGCGCCGATGCCTACAT
CGCTGCAACGGCAGCCGCGCAAGGATTGACCGTTGCCACTCGCGACACGGCACCATTCGAGGCGATGGCGTTAGACGTCA
TTAACCCTTGGAGTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT163007 WP_004150355.1 NZ_CP056216:c5150084-5149842 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT163007 NZ_CP072265:5435143-5435397 [Xanthomonas citri pv. glycines]
ATGCCTTCTTTCACCGTCCGCAATATCCCAGACGACGTGCATCGCGCGATCCGCGCGCGGGCTGCACTTCATGGCCGCAG
CACCGAAGCGGAGATCCGCGCAATCCTTGAATTGGCGGCCAAACCCGCAGACCGGGTCAAACTCGGCTCACTGCTGGTGA
GCATCGGCCGCGACGCCGGCCTGACCGCAAAAGAGGCCAATGCCTTCGACAAGCTGCGCGGCAAGGCGGCGATCAAGCCG
ATCGGTCTGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References