Detailed information of TA system TA163004

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4576737..4577407
• Replicon: chromosome
• Accession: NZ_CP056216
• Organism: Klebsiella pneumoniae strain RHBSTW-00982

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A483GLH7
• Locus tag: HV343_RS22385
• Protein ID: WP_023301398.1
• Coordinates: 4576737..4577069 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A483GII7
• Locus tag: HV343_RS22390
• Protein ID: WP_023301397.1
• Coordinates: 4577090..4577407 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV343_RS22360	4571962..4572634	+	673	Protein_4381	DUF4400 domain-containing protein	-
HV343_RS22365	4572645..4573529	+	885	WP_004192285.1	RES domain-containing protein	-
HV343_RS22370	4573728..4573916	-	189	Protein_4383	transposase	-
HV343_RS22375	4573933..4575044	-	1112	Protein_4384	IS3 family transposase	-
HV343_RS22380	4575443..4576303	-	861	WP_023301399.1	hypothetical protein	-
HV343_RS22385	4576737..4577069	-	333	WP_023301398.1	TA system toxin CbtA family protein	Toxin
HV343_RS22390	4577090..4577407	-	318	WP_023301397.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV343_RS22395	4577426..4577647	-	222	WP_023301396.1	DUF987 domain-containing protein	-
HV343_RS22400	4577656..4578138	-	483	WP_023301395.1	RadC family protein	-
HV343_RS22405	4578147..4578605	-	459	WP_023301394.1	antirestriction protein	-
HV343_RS22410	4578691..4578927	-	237	WP_032446575.1	DUF905 domain-containing protein	-
HV343_RS22415	4579005..4579415	-	411	WP_023301393.1	hypothetical protein	-
HV343_RS22420	4579482..4579919	-	438	WP_023301392.1	hypothetical protein	-
HV343_RS22425	4579961..4580497	-	537	WP_023301391.1	DUF4339 domain-containing protein	-
HV343_RS22430	4580523..4581233	-	711	WP_023301390.1	DeoR family transcriptional regulator	-
HV343_RS22435	4581442..4582266	-	825	WP_023301389.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-101)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12610.65 Da
• Isoelectric Point: 5.6692

>T163004 WP_023301398.1 NZ_CP056216:c4577069-4576737 [Klebsiella pneumoniae]
MKTLPATTPQTAKLCLTSADTWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIPLADAVNFLVDKYALVRIDRRGL
SWQEQSSYLRLVDTQRTIKVIELWLFGVMM

Nucleotide

• Length: 333 bp

>T163004 NZ_CP072265:99562-99840 [Xanthomonas citri pv. glycines]
GTGCCGGCATTGGCTGATCTGGACGCAATTGCCGACTACATCGCAATCGATAACGCACCCGCTGCAGCGGCGCTGGTGAA
ACGGGTGTTCGCGCACGTTGAGCAATTGATCGAACACCCGGACAGCGGAAGCCGACCACAGGAACTCAAGCGCTCGCGCT
ATCGCCAGATTGTCGAACCACCTTGCCGCGTGTTCTACCGCGTGGATGGGCAGCGCATCGTGGTGGTGCACATCATGCGG
TCGGAGCGCGCGCTTCGCGGGAACCGTCTTTCACGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11785.37 Da
• Isoelectric Point: 6.6277

>AT163004 WP_023301397.1 NZ_CP056216:c4577407-4577090 [Klebsiella pneumoniae]
MSNITWGLQRDITPCLGSRLVQEGNRLHYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTLGSSGYVYIAVYLTQR

Nucleotide

• Length: 318 bp

>AT163004 NZ_CP072265:99296-99553 [Xanthomonas citri pv. glycines]
ATGCGCACCGAACTTGTCACCACGCTCAAGCGTCAGGCGACCGAACTCCTTGCAGCTGCAGAGCGCGACAAGGAGCCAAT
CCTGATCACACAGCACGGTTTACCCAGTGCCTATCTGGTGGATGTTGCCAGCTATGAGCGCATGCAGCAGCGCATTGCGC
TCCTCGAAGGAATCGCCCGTGGAGAAATGGCAGTCGCGGAAGGCCGTACGCTGAGCCATGAACAGGCACGGCAACGCATG
GCGCGATGGCTGAAATAA

Structures

Toxin

• AlphaFold DB: A0A483GLH7

Antitoxin

• AlphaFold DB: A0A483GII7