Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4536880..4537690 Replicon chromosome
Accession NZ_CP056216
Organism Klebsiella pneumoniae strain RHBSTW-00982

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag HV343_RS22175 Protein ID WP_023279404.1
Coordinates 4536880..4537413 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HV343_RS22180 Protein ID WP_002887278.1
Coordinates 4537424..4537690 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV343_RS22170 4535711..4536832 + 1122 WP_002887282.1 cupin domain-containing protein -
HV343_RS22175 4536880..4537413 - 534 WP_023279404.1 GNAT family N-acetyltransferase Toxin
HV343_RS22180 4537424..4537690 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
HV343_RS22185 4537793..4539226 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
HV343_RS22190 4539216..4539899 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HV343_RS22195 4540071..4541456 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
HV343_RS22200 4541474..4541818 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T163003 WP_023279404.1 NZ_CP056216:c4537413-4536880 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T163003 NZ_CP072264:c3031-2699 [Xanthomonas citri pv. glycines CFBP 2526]
ATGGAGCGGGGTGATATCTATCTTGTGTCTCTCGATCCTACTTCCGGCCATGAGCAACAGGGCACTCGCCCGGTCCTGAT
TGTGTCGCCGGGACCGTTCAACCGCCTTACCAAAACACCCGTGGTGCTACCGATCACCACGGGTGGCAATTTCGCCCGTA
CGGCAGGTTTCACGGTATCCCTCACGGGGGGAGGCACCAAAACGACCGGAGTCGTGCGCTGCGATCAACCGCGCGCCCTG
GACATTGGATCGCGTAACGGCCGCAAGCTGGAAAGCGTCTCAGCAGCGATCACCGATGAGGTGATGGCTAAGCTGGCGAC
CATACTTGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT163003 WP_002887278.1 NZ_CP056216:c4537690-4537424 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT163003 NZ_CP072264:c3288-3031 [Xanthomonas citri pv. glycines CFBP 2526]
ATGCACACGACAAATCTGCGCAAGGTTGGCGGTTCGGTCATGCTCGCGGTTCCGCCTGCGCTGCTCGATGTGCTGCACAT
CGGGGCAGGGGCAAAAGTGGGCTTGGCTGTCGATAACGGCCGCTTGGTAGTCGAGCCACATGCGCGCCCGCGCTACACCC
TCGCCGACCTGCTCGCCCAATGCGATGCGTCAGCCGAGCTGGCTTCCGAAGATCGGGAATGGCTTGACGCCAAGCCTGTT
GGCGGCGAGCTGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References