Detailed information of TA system TA162987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 224352..225253
• Replicon: plasmid pRHBSTW-00986_2
• Accession: NZ_CP056201
• Organism: Citrobacter sp. RHBSTW-00986

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: HV345_RS25115
• Protein ID: WP_008786589.1
• Coordinates: 224352..224810 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HV345_RS25120
• Protein ID: WP_008786588.1
• Coordinates: 224807..225253 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV345_RS25070	220078..220320	+	243	WP_007372291.1	hypothetical protein	-
HV345_RS25075	220331..220729	+	399	WP_007372292.1	hypothetical protein	-
HV345_RS25080	220813..221139	+	327	WP_007372293.1	hypothetical protein	-
HV345_RS25085	221502..221840	-	339	WP_007372294.1	hypothetical protein	-
HV345_RS25090	222078..222521	+	444	WP_007372295.1	hypothetical protein	-
HV345_RS25095	222640..222981	+	342	WP_007372296.1	hypothetical protein	-
HV345_RS25100	223140..223694	+	555	WP_007372297.1	hypothetical protein	-
HV345_RS25105	223744..224010	+	267	WP_007372298.1	hypothetical protein	-
HV345_RS25110	224078..224308	-	231	WP_007372299.1	hypothetical protein	-
HV345_RS25115	224352..224810	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
HV345_RS25120	224807..225253	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
HV345_RS25125	225402..225719	+	318	WP_016241555.1	hypothetical protein	-
HV345_RS25130	225761..226603	+	843	WP_007372303.1	hypothetical protein	-
HV345_RS25135	226575..226853	+	279	WP_007372304.1	hypothetical protein	-
HV345_RS25140	226913..227356	+	444	WP_007372305.1	hypothetical protein	-
HV345_RS25145	227643..227807	+	165	WP_007372306.1	hypothetical protein	-
HV345_RS25150	228375..229217	+	843	WP_007372307.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..261415	261415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T162987 WP_008786589.1 NZ_CP056201:c224810-224352 [Citrobacter sp. RHBSTW-00986]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T162987 NZ_CP072204:3749960-3750238 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT162987 WP_008786588.1 NZ_CP056201:c225253-224807 [Citrobacter sp. RHBSTW-00986]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT162987 NZ_CP072204:3749697-3749957 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: S1EZ83