Detailed information of TA system TA162986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 217053..217603
• Replicon: plasmid pRHBSTW-00986_2
• Accession: NZ_CP056201
• Organism: Citrobacter sp. RHBSTW-00986

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: HV345_RS25045
• Protein ID: WP_007372286.1
• Coordinates: 217295..217603 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: HV345_RS25040
• Protein ID: WP_007372285.1
• Coordinates: 217053..217292 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV345_RS24995	212060..212455	+	396	WP_024196081.1	hypothetical protein	-
HV345_RS25000	212637..212828	+	192	WP_016241560.1	hypothetical protein	-
HV345_RS25005	212959..213654	+	696	WP_007372278.1	hypothetical protein	-
HV345_RS25010	213677..213874	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
HV345_RS25015	213927..214385	+	459	WP_008786599.1	hypothetical protein	-
HV345_RS25020	214533..214673	+	141	WP_007372281.1	hypothetical protein	-
HV345_RS25025	214689..215321	+	633	WP_007372282.1	hypothetical protein	-
HV345_RS25030	215782..216453	+	672	WP_007372283.1	hypothetical protein	-
HV345_RS25035	216450..216914	+	465	WP_008786596.1	hypothetical protein	-
HV345_RS25040	217053..217292	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HV345_RS25045	217295..217603	+	309	WP_007372286.1	CcdB family protein	Toxin
HV345_RS25050	217624..217782	+	159	WP_016241558.1	hypothetical protein	-
HV345_RS25055	217937..218491	+	555	WP_007372288.1	hypothetical protein	-
HV345_RS25060	218561..219178	+	618	WP_007372289.1	hypothetical protein	-
HV345_RS25065	219261..219731	+	471	WP_007372290.1	hypothetical protein	-
HV345_RS25070	220078..220320	+	243	WP_007372291.1	hypothetical protein	-
HV345_RS25075	220331..220729	+	399	WP_007372292.1	hypothetical protein	-
HV345_RS25080	220813..221139	+	327	WP_007372293.1	hypothetical protein	-
HV345_RS25085	221502..221840	-	339	WP_007372294.1	hypothetical protein	-
HV345_RS25090	222078..222521	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..261415	261415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T162986 WP_007372286.1 NZ_CP056201:217295-217603 [Citrobacter sp. RHBSTW-00986]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T162986 NZ_CP072204:3567481-3568023 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT162986 WP_007372285.1 NZ_CP056201:217053-217292 [Citrobacter sp. RHBSTW-00986]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT162986 NZ_CP072204:3567186-3567497 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3