Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4761210..4761826 Replicon chromosome
Accession NZ_CP056200
Organism Citrobacter sp. RHBSTW-00986

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag HV345_RS22925 Protein ID WP_016155182.1
Coordinates 4761210..4761584 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R8WIS4
Locus tag HV345_RS22930 Protein ID WP_016155181.1
Coordinates 4761584..4761826 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV345_RS22910 4758713..4759615 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV345_RS22915 4759612..4760247 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV345_RS22920 4760244..4761173 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV345_RS22925 4761210..4761584 - 375 WP_016155182.1 type II toxin-antitoxin system VapC family toxin Toxin
HV345_RS22930 4761584..4761826 - 243 WP_016155181.1 ribbon-helix-helix domain-containing protein Antitoxin
HV345_RS22935 4762031..4762960 + 930 WP_181636098.1 alpha/beta hydrolase -
HV345_RS22940 4763045..4763356 + 312 WP_019077936.1 type II toxin-antitoxin system HigB family toxin -
HV345_RS22945 4763353..4763805 + 453 WP_094761751.1 helix-turn-helix domain-containing protein -
HV345_RS22950 4763823..4764764 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV345_RS22955 4764809..4765246 - 438 WP_149382140.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV345_RS22960 4765243..4766115 - 873 WP_181636099.1 virulence factor BrkB family protein -
HV345_RS22965 4766109..4766708 - 600 WP_181636100.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13678.84 Da        Isoelectric Point: 8.5373

>T162984 WP_016155182.1 NZ_CP056200:c4761584-4761210 [Citrobacter sp. RHBSTW-00986]
MVTGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Download         Length: 375 bp

>T162984 NZ_CP072204:2761703-2761810 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9437.72 Da        Isoelectric Point: 4.3331

>AT162984 WP_016155181.1 NZ_CP056200:c4761826-4761584 [Citrobacter sp. RHBSTW-00986]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Download         Length: 243 bp

>AT162984 NZ_CP072204:c2761653-2761590 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB R8WIS4

References