Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3793115..3793794 Replicon chromosome
Accession NZ_CP056200
Organism Citrobacter sp. RHBSTW-00986

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2I8S2T2
Locus tag HV345_RS18435 Protein ID WP_008324514.1
Coordinates 3793453..3793794 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A2I8S2U6
Locus tag HV345_RS18430 Protein ID WP_008324516.1
Coordinates 3793115..3793432 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV345_RS18385 3788556..3789377 + 822 WP_032654809.1 DUF945 domain-containing protein -
HV345_RS18390 3789595..3790296 + 702 WP_032654806.1 WYL domain-containing protein -
HV345_RS18395 3790333..3790569 + 237 WP_001115854.1 hypothetical protein -
HV345_RS18400 3790569..3791012 + 444 WP_001547764.1 phage transcriptional regulator -
HV345_RS18405 3791035..3791502 + 468 WP_001547765.1 hypothetical protein -
HV345_RS18410 3791579..3791818 + 240 WP_000194654.1 DUF905 domain-containing protein -
HV345_RS18415 3791916..3792374 + 459 WP_000211838.1 antirestriction protein -
HV345_RS18420 3792390..3792866 + 477 WP_000811693.1 RadC family protein -
HV345_RS18425 3792875..3793102 + 228 WP_008324517.1 DUF987 domain-containing protein -
HV345_RS18430 3793115..3793432 + 318 WP_008324516.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV345_RS18435 3793453..3793794 + 342 WP_008324514.1 type IV toxin-antitoxin system toxin YkfI Toxin
HV345_RS18445 3794417..3794881 - 465 WP_137367227.1 isoprenylcysteine carboxylmethyltransferase family protein -
HV345_RS18450 3795075..3796523 - 1449 Protein_3618 EAL domain-containing protein -
HV345_RS18455 3796866..3797339 - 474 WP_181635900.1 hypothetical protein -
HV345_RS18460 3797314..3798045 - 732 WP_181635901.1 winged helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12919.98 Da        Isoelectric Point: 9.6543

>T162982 WP_008324514.1 NZ_CP056200:3793453-3793794 [Citrobacter sp. RHBSTW-00986]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T162982 NZ_CP072204:2760632-2760739 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGACGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11787.33 Da        Isoelectric Point: 7.3476

>AT162982 WP_008324516.1 NZ_CP056200:3793115-3793432 [Citrobacter sp. RHBSTW-00986]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Download         Length: 318 bp

>AT162982 NZ_CP072204:c2760585-2760519 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S2T2

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S2U6

References