Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3579733..3580353 Replicon chromosome
Accession NZ_CP056200
Organism Citrobacter sp. RHBSTW-00986

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV345_RS17445 Protein ID WP_002892050.1
Coordinates 3580135..3580353 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV345_RS17440 Protein ID WP_003021733.1
Coordinates 3579733..3580107 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV345_RS17430 3574880..3576073 + 1194 WP_053389373.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV345_RS17435 3576096..3579245 + 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
HV345_RS17440 3579733..3580107 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV345_RS17445 3580135..3580353 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV345_RS17450 3580534..3581085 + 552 WP_016152072.1 maltose O-acetyltransferase -
HV345_RS17455 3581202..3581672 + 471 WP_016152071.1 YlaC family protein -
HV345_RS17460 3581751..3581891 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV345_RS17465 3581893..3582153 - 261 WP_181626390.1 type B 50S ribosomal protein L31 -
HV345_RS17470 3582342..3583895 + 1554 WP_094760921.1 EAL domain-containing protein -
HV345_RS17475 3583947..3584300 - 354 WP_003831033.1 DUF1428 family protein -
HV345_RS17480 3584365..3584994 - 630 WP_103283554.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T162981 WP_002892050.1 NZ_CP056200:3580135-3580353 [Citrobacter sp. RHBSTW-00986]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T162981 NZ_CP072204:c2527865-2527689 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT162981 WP_003021733.1 NZ_CP056200:3579733-3580107 [Citrobacter sp. RHBSTW-00986]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT162981 NZ_CP072204:c2527643-2527227 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References