Detailed information of TA system TA162971

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 920740..921394
• Replicon: chromosome
• Accession: NZ_CP056200
• Organism: Citrobacter sp. RHBSTW-00986

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: HV345_RS04580
• Protein ID: WP_016154348.1
• Coordinates: 920987..921394 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV345_RS04575
• Protein ID: WP_016154349.1
• Coordinates: 920740..921006 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV345_RS04550	915952..917385	-	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-
HV345_RS04555	917507..918235	-	729	WP_008785744.1	MurR/RpiR family transcriptional regulator	-
HV345_RS04560	918288..918599	+	312	WP_101700204.1	N(4)-acetylcytidine aminohydrolase	-
HV345_RS04565	918763..919422	+	660	WP_016154351.1	hemolysin III family protein	-
HV345_RS04570	919502..920482	-	981	WP_019077629.1	tRNA-modifying protein YgfZ	-
HV345_RS04575	920740..921006	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV345_RS04580	920987..921394	+	408	WP_016154348.1	protein YgfX	Toxin
HV345_RS04585	921495..922016	-	522	WP_016154347.1	flavodoxin FldB	-
HV345_RS04590	922129..923025	+	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
HV345_RS04595	923049..923762	+	714	WP_181636306.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV345_RS04600	923768..925501	+	1734	WP_131346809.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T162971 WP_016154348.1 NZ_CP056200:920987-921394 [Citrobacter sp. RHBSTW-00986]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T162971 NZ_CP072204:289207-289734 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT162971 WP_016154349.1 NZ_CP056200:920740-921006 [Citrobacter sp. RHBSTW-00986]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT162971 NZ_CP072204:288934-289200 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6