Detailed information of TA system TA162970

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 696066..696745
• Replicon: chromosome
• Accession: NZ_CP056200
• Organism: Citrobacter sp. RHBSTW-00986

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: J7FU23
• Locus tag: HV345_RS03425
• Protein ID: WP_015063033.1
• Coordinates: 696404..696745 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV345_RS03420
• Protein ID: WP_035893676.1
• Coordinates: 696066..696383 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV345_RS03400	691795..693990	+	2196	WP_035893672.1	DEAD/DEAH box helicase	-
HV345_RS03405	694845..695324	+	480	WP_015063037.1	antirestriction protein	-
HV345_RS03410	695340..695816	+	477	WP_015063036.1	RadC family protein	-
HV345_RS03415	695826..696047	+	222	WP_015063035.1	DUF987 domain-containing protein	-
HV345_RS03420	696066..696383	+	318	WP_035893676.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV345_RS03425	696404..696745	+	342	WP_015063033.1	TA system toxin CbtA family protein	Toxin
HV345_RS03430	697066..698079	-	1014	WP_015386347.1	tyrosine-type recombinase/integrase	-
HV345_RS03435	698306..698920	-	615	WP_181636241.1	phage repressor protein CI	-
HV345_RS03440	699022..699258	+	237	WP_015386350.1	hypothetical protein	-
HV345_RS03445	699293..699802	+	510	WP_015386351.1	phage regulatory CII family protein	-
HV345_RS03450	699810..700010	+	201	WP_015386352.1	DUF2724 domain-containing protein	-
HV345_RS03455	699974..700315	+	342	WP_181636242.1	DUF5347 domain-containing protein	-
HV345_RS03460	700383..700616	+	234	WP_001744223.1	DUF2732 domain-containing protein	-
HV345_RS03465	700616..700843	+	228	WP_181636243.1	TraR/DksA family transcriptional regulator	-
HV345_RS03470	700840..701694	+	855	WP_181636244.1	DNA adenine methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	694845..734347	39502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13051.09 Da
• Isoelectric Point: 9.9961

>T162970 WP_015063033.1 NZ_CP056200:696404-696745 [Citrobacter sp. RHBSTW-00986]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDTGISLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQVTGLLRQSRKNSVR

Nucleotide

• Length: 342 bp

>T162970 NZ_CP072204:267641-267871 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11837.51 Da
• Isoelectric Point: 6.6246

>AT162970 WP_035893676.1 NZ_CP056200:696066-696383 [Citrobacter sp. RHBSTW-00986]
MSNITLGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSEAECLKLDVVFPHFISQMESMLTTGEMNPRHAHCVTLYH
NDFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT162970 NZ_CP072204:267868-268227 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: J7FU23