Detailed information of TA system TA162954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 6437..6962
• Replicon: plasmid pRHBSTW-00995_3
• Accession: NZ_CP056193
• Organism: Klebsiella pneumoniae strain RHBSTW-00995

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: HV347_RS26595
• Protein ID: WP_016946795.1
• Coordinates: 6657..6962 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: HV347_RS26590
• Protein ID: WP_004197642.1
• Coordinates: 6437..6655 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV347_RS26550	1828..2031	-	204	WP_004197809.1	hypothetical protein	-
HV347_RS26555	2045..2248	-	204	WP_004197808.1	hemolysin expression modulator Hha	-
HV347_RS26560	2282..2650	-	369	WP_004197807.1	hypothetical protein	-
HV347_RS26565	2694..3188	-	495	WP_020805591.1	hypothetical protein	-
HV347_RS26570	3219..3791	-	573	WP_020805590.1	hypothetical protein	-
HV347_RS26575	3788..4036	-	249	WP_000272716.1	hypothetical protein	-
HV347_RS26580	4473..5162	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
HV347_RS26585	5194..5883	-	690	WP_004193995.1	hypothetical protein	-
HV347_RS26590	6437..6655	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV347_RS26595	6657..6962	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV347_RS26600	7120..7803	+	684	WP_023280892.1	hypothetical protein	-
HV347_RS26605	7806..8582	+	777	WP_049245386.1	site-specific integrase	-
HV347_RS26610	8640..8897	-	258	WP_000764642.1	hypothetical protein	-
HV347_RS26615	9026..9130	-	105	WP_032409716.1	hypothetical protein	-
HV347_RS26620	9665..10531	+	867	WP_004118283.1	replication initiation protein	-
HV347_RS26625	10888..11157	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..72044	72044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T162954 WP_016946795.1 NZ_CP056193:6657-6962 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T162954 NZ_CP072200:786567-786977 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT162954 WP_004197642.1 NZ_CP056193:6437-6655 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT162954 NZ_CP072200:786320-786586 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA