Detailed information of TA system TA162952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 60467..61203
• Replicon: plasmid pRHBSTW-00995_2
• Accession: NZ_CP056192
• Organism: Klebsiella pneumoniae strain RHBSTW-00995

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: HV347_RS25560
• Protein ID: WP_003026803.1
• Coordinates: 60721..61203 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HV347_RS25555
• Protein ID: WP_003026799.1
• Coordinates: 60467..60733 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV347_RS25515	55830..57368	+	1539	WP_004196907.1	IS66-like element ISEc22 family transposase	-
HV347_RS25520	57658..57921	+	264	WP_009310051.1	hypothetical protein	-
HV347_RS25525	57918..58484	+	567	WP_009310052.1	hypothetical protein	-
HV347_RS25530	58515..59009	+	495	WP_009310053.1	hypothetical protein	-
HV347_RS25535	59053..59421	+	369	WP_009310054.1	hypothetical protein	-
HV347_RS25540	59452..59655	+	204	WP_004098931.1	hemolysin expression modulator Hha	-
HV347_RS25545	59704..59961	+	258	WP_004098928.1	hypothetical protein	-
HV347_RS25550	60037..60291	+	255	WP_004118702.1	hypothetical protein	-
HV347_RS25555	60467..60733	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HV347_RS25560	60721..61203	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
HV347_RS25565	61405..62808	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
HV347_RS25570	62837..63469	-	633	WP_001567369.1	hypothetical protein	-
HV347_RS25575	63699..65045	+	1347	WP_077251107.1	ISNCY family transposase	-
HV347_RS25580	65205..65909	+	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..246880	246880
	-	inside	IScluster/Tn	-	-	55033..73287	18254

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T162952 WP_003026803.1 NZ_CP056192:60721-61203 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T162952 NZ_CP072200:358002-358349 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT162952 WP_003026799.1 NZ_CP056192:60467-60733 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT162952 NZ_CP072200:358349-358648 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGCCCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66