Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 26470..27113 Replicon plasmid pRHBSTW-00995_2
Accession NZ_CP056192
Organism Klebsiella pneumoniae strain RHBSTW-00995

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HV347_RS25370 Protein ID WP_001044770.1
Coordinates 26697..27113 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HV347_RS25365 Protein ID WP_001261282.1
Coordinates 26470..26700 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV347_RS25320 22019..22132 + 114 WP_014343462.1 hypothetical protein -
HV347_RS25325 22261..22518 + 258 WP_009310077.1 hypothetical protein -
HV347_RS25330 22576..23355 - 780 WP_023287113.1 site-specific integrase -
HV347_RS25335 23553..24569 - 1017 WP_009309921.1 hypothetical protein -
HV347_RS25340 24603..24938 - 336 WP_009309920.1 hypothetical protein -
HV347_RS25345 24988..25128 - 141 WP_162898808.1 hypothetical protein -
HV347_RS25350 25357..25662 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB -
HV347_RS25355 25664..25882 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
HV347_RS25360 26052..26513 - 462 WP_075210108.1 hypothetical protein -
HV347_RS25365 26470..26700 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV347_RS25370 26697..27113 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HV347_RS25375 27187..28749 + 1563 WP_009309907.1 AAA family ATPase -
HV347_RS25380 28734..29756 + 1023 WP_000361404.1 helicase UvrD -
HV347_RS25385 30018..30986 + 969 WP_000654814.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..246880 246880
- flank IS/Tn - - 30018..30986 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T162951 WP_001044770.1 NZ_CP056192:26697-27113 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T162951 NZ_CP072200:332031-332399 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GCCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT162951 WP_001261282.1 NZ_CP056192:26470-26700 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT162951 NZ_CP072200:331813-332034 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References