Detailed information of TA system TA162950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 25357..25882
• Replicon: plasmid pRHBSTW-00995_2
• Accession: NZ_CP056192
• Organism: Klebsiella pneumoniae strain RHBSTW-00995

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A7H9GHC5
• Locus tag: HV347_RS25350
• Protein ID: WP_009309918.1
• Coordinates: 25357..25662 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A1D8K3R4
• Locus tag: HV347_RS25355
• Protein ID: WP_006788213.1
• Coordinates: 25664..25882 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV347_RS25315	20519..21487	+	969	WP_000654811.1	IS5 family transposase	-
HV347_RS25320	22019..22132	+	114	WP_014343462.1	hypothetical protein	-
HV347_RS25325	22261..22518	+	258	WP_009310077.1	hypothetical protein	-
HV347_RS25330	22576..23355	-	780	WP_023287113.1	site-specific integrase	-
HV347_RS25335	23553..24569	-	1017	WP_009309921.1	hypothetical protein	-
HV347_RS25340	24603..24938	-	336	WP_009309920.1	hypothetical protein	-
HV347_RS25345	24988..25128	-	141	WP_162898808.1	hypothetical protein	-
HV347_RS25350	25357..25662	-	306	WP_009309918.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV347_RS25355	25664..25882	-	219	WP_006788213.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV347_RS25360	26052..26513	-	462	WP_075210108.1	hypothetical protein	-
HV347_RS25365	26470..26700	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HV347_RS25370	26697..27113	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	-
HV347_RS25375	27187..28749	+	1563	WP_009309907.1	AAA family ATPase	-
HV347_RS25380	28734..29756	+	1023	WP_000361404.1	helicase UvrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..246880	246880
	-	flank	IS/Tn	-	-	30018..30986	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11571.24 Da
• Isoelectric Point: 6.4661

>T162950 WP_009309918.1 NZ_CP056192:c25662-25357 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T162950 NZ_CP072199:c4764637-4764239 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATCAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGCTATGCAGGTAAAATGGTCATTTCGTCGATCACTTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGTCCAG
CGGTGAACGCCAGAGTCGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAATATCCGGGCTGCGCTGGAACGACAGGGAACGCCTATTGGCGTAAACGATCTACATATTGCCGGGCATGCGCGCAG
CGAAGGATTGATCCTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7405

>AT162950 WP_006788213.1 NZ_CP056192:c25882-25664 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT162950 NZ_CP072199:c4764870-4764637 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGACGTTAGGTTCTGTCTTTACTAATAATCGGACACAGGCTGTACGCCTGCCTGCAGAGGCGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTTCGCGTAGTGGGTAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTTCTCAATGAACGTGCCGGACAGCACCAGCGCGAGCGGGAAGCGTTTTGA

Structures

Toxin

• AlphaFold DB: A0A7H9GHC5

Antitoxin

• AlphaFold DB: A0A1D8K3R4