Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5107416..5108041 Replicon chromosome
Accession NZ_CP056191
Organism Klebsiella pneumoniae strain RHBSTW-00995

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HV347_RS24845 Protein ID WP_002882817.1
Coordinates 5107416..5107799 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HV347_RS24850 Protein ID WP_004150355.1
Coordinates 5107799..5108041 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV347_RS24830 5104782..5105684 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HV347_RS24835 5105681..5106316 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HV347_RS24840 5106313..5107242 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HV347_RS24845 5107416..5107799 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HV347_RS24850 5107799..5108041 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HV347_RS24855 5108246..5109163 + 918 WP_004181612.1 alpha/beta hydrolase -
HV347_RS24860 5109177..5110118 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HV347_RS24865 5110163..5110600 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV347_RS24870 5110597..5111457 - 861 WP_023280688.1 virulence factor BrkB family protein -
HV347_RS24875 5111451..5112050 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T162949 WP_002882817.1 NZ_CP056191:c5107799-5107416 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T162949 NZ_CP072199:3694770-3694866 [Serratia marcescens subsp. marcescens ATCC 13880]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT162949 WP_004150355.1 NZ_CP056191:c5108041-5107799 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT162949 NZ_CP072199:c3694866-3694726 [Serratia marcescens subsp. marcescens ATCC 13880]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References