Detailed information of TA system TA162946

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4550693..4551396
• Replicon: chromosome
• Accession: NZ_CP056191
• Organism: Klebsiella pneumoniae strain RHBSTW-00995

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HV347_RS22210
• Protein ID: WP_049190347.1
• Coordinates: 4550693..4551034 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV347_RS22215
• Protein ID: WP_075210061.1
• Coordinates: 4551055..4551396 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV347_RS22195	4546296..4547975	+	1680	WP_032426716.1	glycoside hydrolase family 43 protein	-
HV347_RS22200	4548219..4549088	+	870	WP_142477281.1	HNH endonuclease	-
HV347_RS22205	4549421..4550428	-	1008	WP_001757896.1	restriction endonuclease	-
HV347_RS22210	4550693..4551034	-	342	WP_049190347.1	TA system toxin CbtA family protein	Toxin
HV347_RS22215	4551055..4551396	-	342	WP_075210061.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV347_RS22220	4551407..4551949	-	543	WP_049190342.1	DNA repair protein RadC	-
HV347_RS22225	4551962..4552402	-	441	WP_049190339.1	antirestriction protein	-
HV347_RS22230	4552433..4553254	-	822	WP_049190336.1	DUF945 domain-containing protein	-
HV347_RS22235	4553353..4553583	-	231	WP_049190333.1	DUF905 domain-containing protein	-
HV347_RS22240	4553655..4554104	-	450	WP_014226752.1	hypothetical protein	-
HV347_RS22245	4554101..4554553	-	453	WP_049190329.1	hypothetical protein	-
HV347_RS22250	4554590..4555159	-	570	WP_049190325.1	hypothetical protein	-
HV347_RS22255	4555159..4555863	-	705	WP_049190322.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4550693..4578881	28188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12787.75 Da
• Isoelectric Point: 8.9919

>T162946 WP_049190347.1 NZ_CP056191:c4551034-4550693 [Klebsiella pneumoniae]
MKTLPATNPQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFNDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T162946 NZ_CP072199:1710408-1710683 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGAATTAAAATGGACGAGTAAGGCGCTATCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGCACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTCGATAATCCTCGCCTGGGTGAAAGACTGGATG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATACCCGCGAAGAGCGATAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12710.38 Da
• Isoelectric Point: 6.7345

>AT162946 WP_075210061.1 NZ_CP056191:c4551396-4551055 [Klebsiella pneumoniae]
MKSTDSENDSFLKWGLNRNVAPCFGVRLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT162946 NZ_CP072199:1710148-1710408 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGAATACGAAAGTCATCACCGCTCATGTGCCGCTGCCTTTGGCTGAAAAGGTGGATGAAATGGCCGCCAAACTTGAACG
TTCACGGGGATGGATAGTGAAGCAAGCCCTCGCCGCCTGGCTTGAGCAAGAGGAAGAACGCCGTCGATTAACGCTGGCGG
CGCTGGCCGATGTCGAACAGCAAAACGTTATCGATCATGAGTCGGTAGAAGCCTGGGCTGCCAGCCTGGACAGTGACGAG
CCGCTGCCGGTACCGCGCTGA