Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4522687..4523497 Replicon chromosome
Accession NZ_CP056191
Organism Klebsiella pneumoniae strain RHBSTW-00995

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag HV347_RS22080 Protein ID WP_004178461.1
Coordinates 4522687..4523220 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HV347_RS22085 Protein ID WP_002887278.1
Coordinates 4523231..4523497 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV347_RS22075 4521518..4522639 + 1122 WP_023280822.1 cupin domain-containing protein -
HV347_RS22080 4522687..4523220 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
HV347_RS22085 4523231..4523497 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
HV347_RS22090 4523600..4525033 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
HV347_RS22095 4525023..4525706 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HV347_RS22100 4525879..4527264 + 1386 WP_032412712.1 efflux transporter outer membrane subunit -
HV347_RS22105 4527282..4527626 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T162945 WP_004178461.1 NZ_CP056191:c4523220-4522687 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T162945 NZ_CP072199:1072363-1072674 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGCATATCATTTCCAGGGAACCGTTCAATAACGCAACGAAGCAATATCCCAATGAAGCCACTGCTCTCGAGGCCATTTA
TAAAGCATTGCGACGGGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATCTTGTTCTTTGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAATACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT162945 WP_002887278.1 NZ_CP056191:c4523497-4523231 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT162945 NZ_CP072199:1072671-1073093 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGATTACAGATGCCATTAAGGCGGCCGATGCGCTACTGCGCGCCGTGCCCTTGTTGCAGGGCAGCCGCGCTCAGCAAGA
CTACCGGGAAGCGCTTGAATTGGTGGAATACCTGCTGGAAAACGACGAACACCATCCGCTGATTGACATGCTGGCAAAAA
AGATCGCCGAATATGAAGACAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAGTTACCACAGGGGGTAGCC
GCGCTTCGCGTCCTTATGGAACAGCACCAACTCAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTTAA
CCAAATCCTGAACGGTAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAGCGTTTCAATCTGCCCCCGGCCC
TGTTCCTGCCGGAAGCGGATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References