Detailed information of TA system TA162932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 737142..737920
• Replicon: chromosome
• Accession: NZ_CP056191
• Organism: Klebsiella pneumoniae strain RHBSTW-00995

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HV347_RS03700
• Protein ID: WP_031591140.1
• Coordinates: 737432..737920 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: W8UEW1
• Locus tag: HV347_RS03695
• Protein ID: WP_004150912.1
• Coordinates: 737142..737435 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV347_RS03675	732350..732952	-	603	WP_049190181.1	short chain dehydrogenase	-
HV347_RS03680	733050..733961	+	912	WP_032417598.1	LysR family transcriptional regulator	-
HV347_RS03685	733962..735110	-	1149	WP_032442510.1	PLP-dependent aspartate aminotransferase family protein	-
HV347_RS03690	735121..736497	-	1377	WP_004181304.1	cystathionine beta-synthase	-
HV347_RS03695	737142..737435	+	294	WP_004150912.1	DUF1778 domain-containing protein	Antitoxin
HV347_RS03700	737432..737920	+	489	WP_031591140.1	GNAT family N-acetyltransferase	Toxin
HV347_RS03705	738001..741744	-	3744	Protein_726	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-95)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17604.53 Da
• Isoelectric Point: 7.7754

>T162932 WP_031591140.1 NZ_CP056191:737432-737920 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCGSDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T162932 NZ_CP072172:3853067-3853321 [Rhizobium sp. AB2/73]
ATGAATTCAAAGCAGCGGCAGACGCTGGAAGCCATTTTCAGCAACCCGGTGAAGGCCACGATTAAATGGCAAGACATTGA
GGCCCTGCTGGTGGCAATTGGCTGCGAGATGTCGGAAGGAAGCGGCTCGCGCGTTCGTTTTACGCTAAGCGGCCGAACAT
TGTTCATCCATCGGCCACATCCGAAGCCTGATACGAAGCAATATGTGGTGCGCACGGTCAGGGAGTTTCTGACAGATTTG
GGAATAAAGCCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10901.46 Da
• Isoelectric Point: 6.3783

>AT162932 WP_004150912.1 NZ_CP056191:737142-737435 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Nucleotide

• Length: 294 bp

>AT162932 NZ_CP072172:3853318-3853677 [Rhizobium sp. AB2/73]
ATGAGATCTTACAAGGGATATACCGGTTCGGTGGAATTCGATGAGGATGACATGGTTTTTCATGGCAGAATCGTCGGCAT
CAGAGACATCGTGACTTTTGAAGCGGAAACGGCGCAAGAACTGGTGCAGGCCTTCCGCGACAGCGTCGATGACTACCTCG
CATTCTGCGCGAAAAGATCGCAGGAACCCGATCGACCATTTTCGGGGAATCTTTCCCTGCGCATGACTCCGCGCCTGCAT
GAACAGGTCGTTCGCGCCGCAGGAAACAAGGCTCAGTCCGTCAATCAATGGATCACCGATACTTTGACGGAAGCCGCCTC
TCGAGATGCAGAGTCGGCTTCCATGGTTGTGAAGGCATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GVL4