Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 40081..40634 Replicon plasmid pRHBSTW-01013_3
Accession NZ_CP056187
Organism Citrobacter sp. RHBSTW-01013

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7L6C0D9
Locus tag HV350_RS26285 Protein ID WP_050152488.1
Coordinates 40081..40395 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A7L6C0I8
Locus tag HV350_RS26290 Protein ID WP_050074640.1
Coordinates 40398..40634 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV350_RS26245 36117..36662 - 546 WP_181630777.1 DNA distortion polypeptide 1 -
HV350_RS26250 37001..37258 - 258 WP_050152485.1 hypothetical protein -
HV350_RS26255 37273..37422 - 150 WP_172665488.1 hypothetical protein -
HV350_RS26260 37499..37843 - 345 WP_080994273.1 hypothetical protein -
HV350_RS26265 37939..38157 - 219 WP_181630736.1 hypothetical protein -
HV350_RS26270 38150..38413 - 264 WP_181630784.1 hypothetical protein -
HV350_RS26275 38426..38968 - 543 WP_181630786.1 hypothetical protein -
HV350_RS26280 39010..39525 - 516 WP_128451845.1 J domain-containing protein -
HV350_RS26285 40081..40395 - 315 WP_050152488.1 CcdB family protein Toxin
HV350_RS26290 40398..40634 - 237 WP_050074640.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HV350_RS26295 41657..42562 + 906 WP_181630738.1 replication initiation protein -
HV350_RS26300 42571..43005 + 435 WP_181630740.1 hypothetical protein -
HV350_RS26305 43114..43535 + 422 Protein_53 DNA distortion polypeptide 3 -
HV350_RS26310 44467..44652 + 186 WP_050074651.1 hypothetical protein -
HV350_RS26315 44743..45024 - 282 WP_050074636.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - gnd / galF / manC / manB / gmd 1..73037 73037


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(9-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11747.75 Da        Isoelectric Point: 6.9730

>T162929 WP_050152488.1 NZ_CP056187:c40395-40081 [Citrobacter sp. RHBSTW-01013]
MQFTVYQNTGKTTIYPYLLDVTSDIIGQLNRRIVIPLLPLEKYPTGKRPERMIPIIMLTDGNEYAVMTHELASIPVRALG
AEFCTAVQYRSQVKASIDFLLDGI

Download         Length: 315 bp

>T162929 NZ_CP072172:2208309-2208575 [Rhizobium sp. AB2/73]
ATGTTGCTTGCCTGGCACGCACATGCTTGGGAAGACTATTTATATTGGCGGGAAAGAGATCGTAAAATAGCTGTTAGAAT
CAACGAGCTTTTGCGGGACGCACTACGCCACCCGTTTGAGGGACTCGGCAAACCTGAGGCCCTGCGCAATCAACTCAAAG
GACAATGGTCGCGTCGCATCACGCAAGAGCACAGGCTGATTTACAAAGTCGAAGGTGAAGGCGATCGGCAAATACTTATC
GTTTTGCAATGTCGCTTCCACTATTAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8923.99 Da        Isoelectric Point: 5.8061

>AT162929 WP_050074640.1 NZ_CP056187:c40634-40398 [Citrobacter sp. RHBSTW-01013]
MATKQRNTQSVTMTLERGLLNQAREAGINLSATLVAALDAELRRYAAQKWQKENMEALDALNRFHDENGCFSDEYRTF

Download         Length: 237 bp

>AT162929 NZ_CP072172:2208041-2208301 [Rhizobium sp. AB2/73]
ATGCGAACGGTCATGTTTTCAGCAGCACGAAATGAACTTGCTAGCTTGCTTGACGAAGTCGTGCAGGACCGTGTTGCCGT
GGAAATTGTTCGTCGCGATAAGCCGTCAGCCATTCTGATCGACAAGGAAGAATATGAAGGTATCATCGAGACGCTTCATG
TCCTGTCGACCCCGGCCAATGCGGCGCGGTTGATGGAAGCGATTACCGATGCAAACGAGGGAAAGAACCTCGTTTCTCAC
GATTTGATTGATCCGAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6C0D9

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6C0I8

References