Detailed information of TA system TA162927

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4453110..4453836
• Replicon: chromosome
• Accession: NZ_CP056185
• Organism: Citrobacter sp. RHBSTW-01013

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV350_RS21355
• Protein ID: WP_094760975.1
• Coordinates: 4453110..4453451 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV350_RS21360
• Protein ID: WP_094760976.1
• Coordinates: 4453495..4453836 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV350_RS21320	4448612..4448938	-	327	WP_149382327.1	DUF1493 family protein	-
HV350_RS21325	4448932..4449381	-	450	WP_137367027.1	hypothetical protein	-
HV350_RS21330	4449640..4450512	+	873	WP_181629283.1	HNH endonuclease	-
HV350_RS21335	4450647..4451441	-	795	WP_094760971.1	helix-turn-helix transcriptional regulator	-
HV350_RS21340	4451557..4451832	-	276	WP_094760972.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HV350_RS21345	4451836..4452084	-	249	WP_094760973.1	ribbon-helix-helix domain-containing protein	-
HV350_RS21350	4452161..4452994	-	834	WP_094760974.1	DUF4942 domain-containing protein	-
HV350_RS21355	4453110..4453451	-	342	WP_094760975.1	TA system toxin CbtA family protein	Toxin
HV350_RS21360	4453495..4453836	-	342	WP_094760976.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV350_RS21365	4453859..4454080	-	222	WP_094760977.1	DUF987 family protein	-
HV350_RS21370	4454077..4454586	-	510	WP_171862414.1	DNA repair protein RadC	-
HV350_RS21375	4454598..4455017	-	420	WP_094760978.1	antirestriction protein	-
HV350_RS21380	4455131..4455949	-	819	WP_094760979.1	DUF945 domain-containing protein	-
HV350_RS21385	4456066..4456296	-	231	WP_094760980.1	hypothetical protein	-
HV350_RS21390	4456293..4456526	-	234	WP_094760981.1	DUF905 domain-containing protein	-
HV350_RS21395	4456632..4456907	-	276	WP_094760982.1	hypothetical protein	-
HV350_RS21400	4457181..4458038	-	858	WP_094760983.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4448612..4483253	34641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-107)

Antitoxin

• Antitoxin CbeA (11-109)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12990.86 Da
• Isoelectric Point: 9.7617

>T162927 WP_094760975.1 NZ_CP056185:c4453451-4453110 [Citrobacter sp. RHBSTW-01013]
MQTQYNHPYRATPSQPSPVEIWQKMLTHLLAKHYGLTLNDTPFSEEKVIQEHIDAGITLANAVNFIVEKYELVRIDRKGF
GWQDASPYLRAVDILRARQATGLLRGRRHLAAH

Nucleotide

• Length: 342 bp

>T162927 NZ_CP072172:c2037404-2036994 [Rhizobium sp. AB2/73]
ATGTATCTCGTCGATACCAACGTCGTCTCGGAACTCCGGCGCGGCAATTCCCAAGCGATTGCGTGGCTGCGCTCGGTCGA
TCCCTCCACCATTTATCTGAGTGTCATCACACTCGGAGAGATCATGCGTGGGATTGCGCTCAAGCGAAAATCGGACCCGC
GTACCGCAACACATCTGGAGGAATGGTTGCGCAAGCTCCGGCACGACCATCGCAATCGCATTCTTCCCATTACCGATCAG
ATTGCGGTCGAATGGGGGCGTATCGCGGCACAGCGACCGCGTGGTGACGCAGATGGGCTCATCGCGGCAACGGCGATCGT
TCATGACCTTATCGTCGTCACTCGCAACATCGGCGATTTCGACGACACCGGCGCCTTAATCGTCAACCCCTGGGATCAGA
AGCCTTACTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12705.72 Da
• Isoelectric Point: 9.7668

>AT162927 WP_094760976.1 NZ_CP056185:c4453836-4453495 [Citrobacter sp. RHBSTW-01013]
MKINKTTSTPVWGLRKDVFPSFGARLVQEGNHLHYLADRAGIYGEFTPAQLKTLNIVFPMFIKQMEAALRTGALNPREAR
RFTTEIRGITCEADTCGSFGYVYLSLYPTIVKS

Nucleotide

• Length: 342 bp

>AT162927 NZ_CP072172:c2037658-2037404 [Rhizobium sp. AB2/73]
ATGAATTGGCCATTGCAGGATGCAAAGAATCAGTTTTCCAAAGTCGTACAGAAAGCACGTAGCGAAGGGCCGCAAATCGT
CACCTTGCGCGGCGAACCCGCGGCGGTCGTTCTGTCTGCCGAAGACTATGATGCACTTCTGGCTGGCAAACCGAGCCTTG
CCGATGATCTGCTGTCGGGGCCGGCTTGGGACGACGAAATAGCCGAAGCCGTATCTAAGCGAGATAAAACGCCAAGCCGG
GATGTCCTTTTCTGA