Detailed information of TA system TA162925

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 4012463..4013142
• Replicon: chromosome
• Accession: NZ_CP056185
• Organism: Citrobacter sp. RHBSTW-01013

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A2I8S2T2
• Locus tag: HV350_RS19310
• Protein ID: WP_008324514.1
• Coordinates: 4012801..4013142 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A2I8S2U6
• Locus tag: HV350_RS19305
• Protein ID: WP_008324516.1
• Coordinates: 4012463..4012780 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV350_RS19260	4007904..4008725	+	822	WP_032654809.1	DUF945 domain-containing protein	-
HV350_RS19265	4008943..4009644	+	702	WP_008324530.1	WYL domain-containing protein	-
HV350_RS19270	4009681..4009917	+	237	WP_001115854.1	hypothetical protein	-
HV350_RS19275	4009917..4010360	+	444	WP_001547764.1	phage transcriptional regulator	-
HV350_RS19280	4010383..4010850	+	468	WP_001547765.1	hypothetical protein	-
HV350_RS19285	4010927..4011166	+	240	WP_000194654.1	DUF905 domain-containing protein	-
HV350_RS19290	4011264..4011722	+	459	WP_000211838.1	antirestriction protein	-
HV350_RS19295	4011738..4012214	+	477	WP_000811693.1	RadC family protein	-
HV350_RS19300	4012223..4012450	+	228	WP_008324517.1	DUF987 domain-containing protein	-
HV350_RS19305	4012463..4012780	+	318	WP_008324516.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV350_RS19310	4012801..4013142	+	342	WP_008324514.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HV350_RS19320	4013765..4014229	-	465	WP_181629146.1	isoprenylcysteine carboxylmethyltransferase family protein	-
HV350_RS19325	4014423..4015871	-	1449	WP_181629147.1	EAL domain-containing protein	-
HV350_RS19330	4016214..4016687	-	474	WP_141874847.1	hypothetical protein	-
HV350_RS19335	4016662..4017393	-	732	WP_141874846.1	winged helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3987619..4025801	38182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12919.98 Da
• Isoelectric Point: 9.6543

>T162925 WP_008324514.1 NZ_CP056185:4012801-4013142 [Citrobacter sp. RHBSTW-01013]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T162925 NZ_CP072172:469832-470236 [Rhizobium sp. AB2/73]
GTGAAGTATCTGCTCGACAGCAATGCGATCATCGCACTGATGAAAGGGCACCCCGGCTTCGTCGGCAAGCTTCGCAGGCA
CAAACCGCAGGATTTTGCCATTCCGGCCATCGTCGCACACGAGCTGATTTATGGTGCCTATAAGGGGCAGCGCGTTTCCG
ACAATCTGGCACGTGTCGATGCCCTGCAATTCGAGACGCTGGATTTCGATAAGCAAGATGCCCGTGTAGCCGGCGAAATT
CGCGCAAGCCTTGCTTCCCTCGGCACACCGATCGGTGCCTATGACGTTTTGATCGCGGGGCAAGCGGTCGCCCGCGATCT
GATCCTGATAACGCACAATGTCAAAGAATTTGGACGCGTCCCCAAGCTGCGTTTCGAAGACTGGGAGTCCTTGCCGAGCG
GCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11787.33 Da
• Isoelectric Point: 7.3476

>AT162925 WP_008324516.1 NZ_CP056185:4012463-4012780 [Citrobacter sp. RHBSTW-01013]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT162925 NZ_CP072172:469593-469835 [Rhizobium sp. AB2/73]
ATGGACACCGCAAAGATCTTCTGGTCCGGCCGCTCGCAGGCCGTACGCTTGCCGAAGGAATTCCGCCTCGATGGCGAGGA
AGTCCGCATACGCCGGCAGGGAAAGGCAATCATTCTGGAGCCGATCACCGAAGATTGGGACTGGCTGGAAGCGGTAACAG
GCCCGGTCGACGATGACTTTGTAGAAGCAGCGACCGAGCAACCGGCTGAGCAGCAACGCCCCGAGTTGGATATCTTCAAG
TGA

Structures

Toxin

• AlphaFold DB: A0A2I8S2T2

Antitoxin

• AlphaFold DB: A0A2I8S2U6