Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3749685..3750305 Replicon chromosome
Accession NZ_CP056185
Organism Citrobacter sp. RHBSTW-01013

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV350_RS18085 Protein ID WP_002892050.1
Coordinates 3750087..3750305 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A7L6U5G9
Locus tag HV350_RS18080 Protein ID WP_019076175.1
Coordinates 3749685..3750059 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV350_RS18070 3744832..3746025 + 1194 WP_016152074.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV350_RS18075 3746048..3749197 + 3150 WP_016152073.1 multidrug efflux RND transporter permease subunit -
HV350_RS18080 3749685..3750059 + 375 WP_019076175.1 Hha toxicity modulator TomB Antitoxin
HV350_RS18085 3750087..3750305 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV350_RS18090 3750486..3751037 + 552 WP_181629072.1 maltose O-acetyltransferase -
HV350_RS18095 3751154..3751624 + 471 WP_016152071.1 YlaC family protein -
HV350_RS18100 3751703..3751843 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV350_RS18105 3751845..3752105 - 261 WP_016152070.1 type B 50S ribosomal protein L31 -
HV350_RS18110 3752294..3753846 + 1553 Protein_3552 EAL domain-containing protein -
HV350_RS18115 3753898..3754251 - 354 WP_003831033.1 DUF1428 family protein -
HV350_RS18120 3754316..3754945 - 630 WP_016155872.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T162924 WP_002892050.1 NZ_CP056185:3750087-3750305 [Citrobacter sp. RHBSTW-01013]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T162924 NZ_CP072172:74199-74468 [Rhizobium sp. AB2/73]
GTGGCATGGACGATTGAGTATGCGCGATCTGCTCGCAAATTTGTCGAAAAACTGAATCCGGAGACACGTAAGAGAATAAG
GAGTTTTCTCGAGGAACGTCTCGCCACTTTGCATGATCCCCGTCAGGTTGGCGATGCTCTGCAGGGTGCGCGGTTGGGGA
ATTACTGGCGTTATCGAGTCGGCGATTACCGAATTATCTGCGACCTGCAGGATAGTCGCCTGGTTGTGCTTGTTATCGAA
ATCGGAAACCGTCGCGACGTCTATCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14413.20 Da        Isoelectric Point: 5.5653

>AT162924 WP_019076175.1 NZ_CP056185:3749685-3750059 [Citrobacter sp. RHBSTW-01013]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLVAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT162924 NZ_CP072172:73988-74215 [Rhizobium sp. AB2/73]
ATGAGCAAACAAACCGCCATCCGTCTGCCAGATGAAACCTACGAGCGCCTGCAGGCTCTGGCCACGCGAACGGGCCGGAC
CGCAACCTTCTACATTCGTCAAGCCGTTGAGGCTCACTTGGACGAAATGGAAGACGTATACTTAGCAGAACGCGCCATGG
AGCGAATTAAGAACGGTGAAGACGAGGTCGTAAGCTCCGAGGAATTCTGGCGTGGCATGGACGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6U5G9

References