Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 3087955..3088545 Replicon chromosome
Accession NZ_CP056185
Organism Citrobacter sp. RHBSTW-01013

Toxin (Protein)


Gene name pemK Uniprot ID A0A7L6BU22
Locus tag HV350_RS14950 Protein ID WP_049282111.1
Coordinates 3087955..3088287 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A7L6BU61
Locus tag HV350_RS14955 Protein ID WP_049282110.1
Coordinates 3088288..3088545 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV350_RS14925 3082991..3083350 - 360 WP_016156204.1 purine nucleoside phosphoramidase -
HV350_RS14930 3083699..3085885 + 2187 WP_181628813.1 ferric-rhodotorulic acid/ferric-coprogen receptor FhuE -
HV350_RS14940 3086064..3087707 + 1644 WP_181628814.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
HV350_RS14950 3087955..3088287 - 333 WP_049282111.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HV350_RS14955 3088288..3088545 - 258 WP_049282110.1 antitoxin Antitoxin
HV350_RS14960 3089157..3093056 + 3900 Protein_2940 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11758.56 Da        Isoelectric Point: 8.5572

>T162923 WP_049282111.1 NZ_CP056185:c3088287-3087955 [Citrobacter sp. RHBSTW-01013]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGVGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILN

Download         Length: 333 bp

>T162923 NZ_CP072168:c283468-283175 [Agrobacterium larrymoorei]
TTGAGACTAACGTGGTCCGCGTTTGCCTTATCGGATCGCGACACAATTTTTACTTTCATCGAGTTGGAGAACGCGGCCGC
TGCCATTTTGGTCGACGAACGTATCGTCGTTGCAGTCCGTCGCCTGATCGATTTTCCAGCAAGTGGCAGGGCCGGAAGAA
TTGCTGGGACACGTGAACTTGTAATCAGCGGAACGCCTTACGTCGCAGTATATACGATCACAGAAGCTACGGTTCGAGTA
CTGCGTATTTTGCACGGGGCTCAGGAATGGCCTGAAGCTTTTTCAATCAGTTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9398.91 Da        Isoelectric Point: 4.4620

>AT162923 WP_049282110.1 NZ_CP056185:c3088545-3088288 [Citrobacter sp. RHBSTW-01013]
MYTTRLKKVGGSIMLAVPPAVLKTLALSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEEREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT162923 NZ_CP072168:c283773-283465 [Agrobacterium larrymoorei]
ATGGCTGCAAATGCGCTCGTTCAAACTCGTATCGATGCTGACGTTCGTGACCGGGCGTCCCTCGTTCTGGAAGGTATGGG
ACTTACGGTGTCAGACGCGGTTCGCATATTGCTTACGAGGACCGCCAATGAGGGCATGTTGCCGCTTGAACTTCTTTCAG
GCAGCGAGGCTCACGATACCTGGTTTCGTGCAAAGGTGCTTGAGGCGCTAAATGACACAAGACCGGATATTTCCGACGAT
GAAGTGGAAGCCCATTTCGAAAAGCGTCGGCATTCTGCGCGCAATAACGCGGCGGGTATAACGTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6BU22

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6BU61

References