Detailed information of TA system TA162914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2569643..2570279
• Replicon: chromosome
• Accession: NZ_CP056185
• Organism: Citrobacter sp. RHBSTW-01013

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HV350_RS12460
• Protein ID: WP_181627388.1
• Coordinates: 2569643..2569831 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV350_RS12465
• Protein ID: WP_131341011.1
• Coordinates: 2569863..2570279 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV350_RS12440	2566421..2566651	-	231	WP_016153158.1	DUF2554 family protein	-
HV350_RS12445	2566841..2566966	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV350_RS12450	2566966..2567976	-	1011	WP_181630589.1	cytochrome d ubiquinol oxidase subunit II	-
HV350_RS12455	2567976..2569379	-	1404	WP_016153156.1	cytochrome ubiquinol oxidase subunit I	-
HV350_RS12460	2569643..2569831	+	189	WP_181627388.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV350_RS12465	2569863..2570279	+	417	WP_131341011.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV350_RS12470	2570372..2571781	+	1410	WP_181630590.1	PLP-dependent aminotransferase family protein	-
HV350_RS12475	2572111..2573256	+	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
HV350_RS12480	2573273..2574289	+	1017	WP_016156582.1	ABC transporter ATP-binding protein	-
HV350_RS12485	2574290..2575234	+	945	WP_016153152.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7174.22 Da
• Isoelectric Point: 11.2510

>T162914 WP_181627388.1 NZ_CP056185:2569643-2569831 [Citrobacter sp. RHBSTW-01013]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQERRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T162914 NZ_CP072167:829654-829815 [Agrobacterium larrymoorei]
ATGAAGAGCACTGATATTATAGCGGCACTTCGCAAGGGCAGTCATGTCCAGTTCAAGCATGCTTCGAAACCCGGACGTGT
CACCGTCCCGCATCCCAAGAGAGACTTGCCCATCGGTACGCTTAAAAGCATAGAGAAGCAGGCCGGGTTGAAACTGAGGT
AG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15034.31 Da
• Isoelectric Point: 4.7119

>AT162914 WP_131341011.1 NZ_CP056185:2569863-2570279 [Citrobacter sp. RHBSTW-01013]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGAEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT162914 NZ_CP072167:829820-830221 [Agrobacterium larrymoorei]
ATGCGGAATTACATCGGACTGATCCACAAGCACGCTGATAGCGATTACGGAGTATCGTTCCCCGACTTCCCAGGCGTCGT
GACTGCTGGAACGGATTTGGATGACGCACGTCTGATGGCCGAGGAAGCGCTATCCCTTCATGTCGAGGGGCTGCTGGAAG
ATGGCGACGCCATTCCCGAGCCATCGAGTCTGGATGACGTTATGAAAGATGGCGAAAACAAAAAGGCTGTCGCAATCCTC
GTTTCGCTGAAGTCCGAGAGCAAGAGAGCCGTTCGCCTTAACATCACCTTGCCTGAAGACGTTCTGCGGGACATCGATGC
TTACGCACAAGCGCACGGATTGACCCGTTCGGGCTTCCTGGCGCGCGCCGCGAAACACGAAATCTCCTCTGCGAGTGCCT
GA