Detailed information of TA system TA162912

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1252940..1253607
• Replicon: chromosome
• Accession: NZ_CP056185
• Organism: Citrobacter sp. RHBSTW-01013

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HV350_RS06025
• Protein ID: WP_181630016.1
• Coordinates: 1252940..1253269 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A0F6RHY7
• Locus tag: HV350_RS06030
• Protein ID: WP_046495841.1
• Coordinates: 1253290..1253607 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV350_RS06000	1248360..1249793	+	1434	WP_181630010.1	EAL domain-containing protein	-
HV350_RS06005	1249852..1250208	-	357	WP_016157245.1	hypothetical protein	-
HV350_RS06010	1250664..1251353	+	690	WP_181630012.1	YafY family transcriptional regulator	-
HV350_RS06015	1251424..1252170	+	747	WP_181630014.1	SDR family oxidoreductase	-
HV350_RS06020	1252361..1252543	-	183	WP_181630015.1	hypothetical protein	-
HV350_RS06025	1252940..1253269	-	330	WP_181630016.1	TA system toxin CbtA family protein	Toxin
HV350_RS06030	1253290..1253607	-	318	WP_046495841.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV350_RS06035	1253626..1253847	-	222	WP_000691995.1	DUF987 domain-containing protein	-
HV350_RS06040	1253856..1254332	-	477	WP_046495838.1	RadC family protein	-
HV350_RS06045	1254348..1254806	-	459	WP_128347215.1	antirestriction protein	-
HV350_RS06050	1254890..1255129	-	240	WP_054626379.1	DUF905 domain-containing protein	-
HV350_RS06055	1255229..1256137	-	909	WP_064761968.1	hypothetical protein	-
HV350_RS06060	1256206..1256373	-	168	Protein_1187	DUF4339 domain-containing protein	-
HV350_RS06065	1256411..1257106	-	696	WP_054626378.1	hypothetical protein	-
HV350_RS06070	1257333..1258160	-	828	WP_054626377.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1250664..1278352	27688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12392.27 Da
• Isoelectric Point: 8.9791

>T162912 WP_181630016.1 NZ_CP056185:c1253269-1252940 [Citrobacter sp. RHBSTW-01013]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRNGF
SWQEQTPYISVVDILRARRSTGLLKNNVK

Nucleotide

• Length: 330 bp

>T162912 NZ_CP072167:328501-328797 [Agrobacterium larrymoorei]
ATGAAAGTAGAAGTGACCGATTGGGATACGAGCGATTACCTCAATGGCAATGAAGCCGTGATCGCCTATCTGGAAGCCTC
GTTCGAGGATGGCGATCCGAAGGTCATTGCCATGGCCCTCGGCAACATCGCGAAAGCCAAAGGCATGAGCCAGGTCGCCA
AGCAAGCGGGCATTACCCGGGAAGCGCTTTATAAGTCGCTCAGTGAAAAGGGCGACCCCAAGCTTTCGACCCTCATCGGA
GTGATGAAGGCGTTAGGCTTACGTCTTTCGGTCGCCCCTGAAGAAAGAGCCGCGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11869.49 Da
• Isoelectric Point: 6.6245

>AT162912 WP_046495841.1 NZ_CP056185:c1253607-1253290 [Citrobacter sp. RHBSTW-01013]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Nucleotide

• Length: 318 bp

>AT162912 NZ_CP072167:328212-328499 [Agrobacterium larrymoorei]
ATGATCAGAATTCGGAAAACGAATATCTTCTCGGACTGGTTTACGTCCCTGCGAGATAAAAGAGCGCAAGCAAGGATTCA
GGTTCGTATTGACCGTCTTTCGCTGGGACTTCTCGGCGACGTGAAATTTTTCGATGGCATTGGCGAAATGCGGATCGATT
ACGGGCCGGGCTACCGGGTTTATTTCACGCGAAGGCAAAACGAGATAGTTATTCTGCTGTGCGGTGGAGACAAGGGATCT
CAGACGAGAGACATCGAACGCGCCATCAAGATTGCAGCCGAGGTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A0F6RHY7