Detailed information of TA system TA162911

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 926985..927639
• Replicon: chromosome
• Accession: NZ_CP056185
• Organism: Citrobacter sp. RHBSTW-01013

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: HV350_RS04570
• Protein ID: WP_016154348.1
• Coordinates: 927232..927639 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV350_RS04565
• Protein ID: WP_016154349.1
• Coordinates: 926985..927251 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV350_RS04540	922198..923631	-	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-
HV350_RS04545	923752..924480	-	729	WP_003838265.1	MurR/RpiR family transcriptional regulator	-
HV350_RS04550	924533..924844	+	312	WP_181629884.1	N(4)-acetylcytidine aminohydrolase	-
HV350_RS04555	925008..925667	+	660	WP_181629885.1	hemolysin III family protein	-
HV350_RS04560	925747..926727	-	981	WP_103928567.1	tRNA-modifying protein YgfZ	-
HV350_RS04565	926985..927251	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV350_RS04570	927232..927639	+	408	WP_016154348.1	protein YgfX	Toxin
HV350_RS04575	927746..928267	-	522	WP_181629887.1	flavodoxin FldB	-
HV350_RS04580	928381..929277	+	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
HV350_RS04585	929301..930014	+	714	WP_181629889.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV350_RS04590	930020..931753	+	1734	WP_016157413.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T162911 WP_016154348.1 NZ_CP056185:927232-927639 [Citrobacter sp. RHBSTW-01013]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T162911 NZ_CP072167:328212-328499 [Agrobacterium larrymoorei]
ATGATCAGAATTCGGAAAACGAATATCTTCTCGGACTGGTTTACGTCCCTGCGAGATAAAAGAGCGCAAGCAAGGATTCA
GGTTCGTATTGACCGTCTTTCGCTGGGACTTCTCGGCGACGTGAAATTTTTCGATGGCATTGGCGAAATGCGGATCGATT
ACGGGCCGGGCTACCGGGTTTATTTCACGCGAAGGCAAAACGAGATAGTTATTCTGCTGTGCGGTGGAGACAAGGGATCT
CAGACGAGAGACATCGAACGCGCCATCAAGATTGCAGCCGAGGTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT162911 WP_016154349.1 NZ_CP056185:926985-927251 [Citrobacter sp. RHBSTW-01013]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT162911 NZ_CP072167:328501-328797 [Agrobacterium larrymoorei]
ATGAAAGTAGAAGTGACCGATTGGGATACGAGCGATTACCTCAATGGCAATGAAGCCGTGATCGCCTATCTGGAAGCCTC
GTTCGAGGATGGCGATCCGAAGGTCATTGCCATGGCCCTCGGCAACATCGCGAAAGCCAAAGGCATGAGCCAGGTCGCCA
AGCAAGCGGGCATTACCCGGGAAGCGCTTTATAAGTCGCTCAGTGAAAAGGGCGACCCCAAGCTTTCGACCCTCATCGGA
GTGATGAAGGCGTTAGGCTTACGTCTTTCGGTCGCCCCTGAAGAAAGAGCCGCGTAA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6