Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 728829..729508 Replicon chromosome
Accession NZ_CP056185
Organism Citrobacter sp. RHBSTW-01013

Toxin (Protein)


Gene name ykfI Uniprot ID A0A7D6Z078
Locus tag HV350_RS03610 Protein ID WP_048215514.1
Coordinates 729167..729508 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0F6RHY7
Locus tag HV350_RS03605 Protein ID WP_046495841.1
Coordinates 728829..729146 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV350_RS03565 724388..725221 + 834 WP_048215513.1 DUF945 domain-containing protein -
HV350_RS03570 725423..726127 + 705 WP_046495813.1 WYL domain-containing protein -
HV350_RS03575 726124..726738 + 615 WP_046495819.1 hypothetical protein -
HV350_RS03580 726827..727237 + 411 WP_046495825.1 hypothetical protein -
HV350_RS03585 727315..727551 + 237 WP_046495830.1 DUF905 domain-containing protein -
HV350_RS03590 727630..728088 + 459 WP_046495834.1 antirestriction protein -
HV350_RS03595 728104..728580 + 477 WP_046495838.1 RadC family protein -
HV350_RS03600 728589..728810 + 222 WP_000691995.1 DUF987 domain-containing protein -
HV350_RS03605 728829..729146 + 318 WP_046495841.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV350_RS03610 729167..729508 + 342 WP_048215514.1 TA system toxin CbtA family protein Toxin
HV350_RS03615 729624..730457 + 834 WP_046495848.1 DUF4942 domain-containing protein -
HV350_RS03625 730761..731267 + 507 WP_103767095.1 G/U mismatch-specific DNA glycosylase -
HV350_RS03630 731313..733157 - 1845 WP_016154549.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 702194..735067 32873


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(2-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13014.97 Da        Isoelectric Point: 10.4182

>T162910 WP_048215514.1 NZ_CP056185:729167-729508 [Citrobacter sp. RHBSTW-01013]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRRRF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T162910 NZ_CP072167:c158782-158489 [Agrobacterium larrymoorei]
ATGCCACAACTGATCTGGACGCCAGAAGCACTCAGGGACGTTCAGCGCTGTTATCGTTTTCTTGCCCCGAAAAATCCAGA
TGCTGCATCTCGGGCGATAAAGTCCATTCGCGAAAAGATGCGGATGATCGTGACGCATCCGGCCATTGGCCGCCCCGTCG
ACAAGATGGCCCCGGAATTTCGGGAATTGCTTATTCCGTTTGGTGAGAGCGGCTATGTCGCGCTTTACCGTTTGGATGGT
GATCTGGCCGTGGTTTTGGCCGTGCGTCATCAAAATGAAGCTGGTTACACCTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11869.49 Da        Isoelectric Point: 6.6245

>AT162910 WP_046495841.1 NZ_CP056185:728829-729146 [Citrobacter sp. RHBSTW-01013]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Download         Length: 318 bp

>AT162910 NZ_CP072167:c159054-158770 [Agrobacterium larrymoorei]
ATGCCGGGACCACATCCAGTTGCGGTGAAGCTCGATCCGGAGGTCCACGCCCGGATGCGGAAGCTTGCGCAGGCGCAGCA
CCGTTCCACGCACTACCTGATGCGCGAGGCTATCGCGCAATATGTTGAGCGTGAGGAAAAGCGTGCGGCGTTTCGGCAGG
ATGCTTTGGAGGCGTGGTCTGCTTATCAGGCGAGTGGATCGCATCTCAATCAGACAGAAGCCGATGAGTGGTTGGCCAAG
CTTGAGGCCGGGCAGGACGTGGAACCGCCTGAATGCCACAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6Z078

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6RHY7

References