Detailed information of TA system TA162902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2945133..2945723
• Replicon: chromosome
• Accession: NZ_CP056180
• Organism: Citrobacter sp. RHBSTW-01044

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HV352_RS14375
• Protein ID: WP_049041157.1
• Coordinates: 2945133..2945465 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: HV352_RS14380
• Protein ID: WP_049041154.1
• Coordinates: 2945466..2945723 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV352_RS14350	2940169..2940528	-	360	WP_016156204.1	purine nucleoside phosphoramidase	-
HV352_RS14355	2940877..2943063	+	2187	WP_181634828.1	ferric-rhodotorulic acid/ferric-coprogen receptor FhuE	-
HV352_RS14365	2943242..2944885	+	1644	WP_181634829.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HV352_RS14375	2945133..2945465	-	333	WP_049041157.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HV352_RS14380	2945466..2945723	-	258	WP_049041154.1	antitoxin	Antitoxin
HV352_RS14385	2946335..2950234	+	3900	WP_109017714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11731.54 Da
• Isoelectric Point: 8.5572

>T162902 WP_049041157.1 NZ_CP056180:c2945465-2945133 [Citrobacter sp. RHBSTW-01044]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPASFNKFTRLPVVVPVTSGGNFARAAGFTVSLDGVGTKTTGVIRCDQPRTI
DMGARNGKCLERIPEAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T162902 NZ_CP072162:c2998283-2998038 [Rhizobium sp. B21/90]
ATGAAGGACGTTGTCGTATCCCGACTTGCCGACAAAGCACTTATGCGCATGCAGCCAAAACGGCGGCTGGCGATTATTGA
AAAAGTCAAAGCCTATGCGCGCGGAGAGATTGTCGACATCAAGAAGATGAAAGGCGGAAATCTCTACCGTATCAGGGTCG
GACAGGATCGCGTCATCATCGACGACAAGGGTAGGATTGTCGTGGTCATCGATGCCGGTCCGCGGGGCAGTATCTACAAG
GAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9370.85 Da
• Isoelectric Point: 4.4354

>AT162902 WP_049041154.1 NZ_CP056180:c2945723-2945466 [Citrobacter sp. RHBSTW-01044]
MYTTRLKKVGGSIMLAVPPAVLKTLALSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT162902 NZ_CP072162:c2998031-2997669 [Rhizobium sp. B21/90]
ATGGGCGATATCAAGAGGTTCGAAATCGACGGCAAACCCTATGTGCTGTTGAGTGAAGAGGATTATGAGGATCTCGTCGA
TGGACTGAGGGCGAATGCTGTCCTGGCCGGGATTTCTGCAGGAGAAGAAACCTGGCCCTTGGAGATCGTTGAGGCGCGTG
CCAATGGCGGAAATGCCGTTCGTATCTTCCGAAACTATCGCCGCATGACTGTTACCGAACTTGCCACGGCCGCCGGAATT
TCGCAGCCCTATCTCTCTGAGATCGAGGCAGGCAAAAAAACCGGCAGTGTCGATGTCCTGAAGCGTATCGCTAACGCCCT
CAAGGTCGATCTCGACGATCTCGTCGTCAACGTGGCGGACTGA